- ￥1350 - 3000
- Proteintech
- 10213-2-AP
- 2025年10月13日
- WB, IHC, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human RABEPK protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, ELISA
- 靶点：
RABEPK/p40
- 抗体英文名：
RABEPK/p40 Polyclonal antibody
- 抗体名：
RABEPK/p40 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HepG2 cells, K-562 cells, HeLa cells |
| Positive IHC detected in | human lung squamous cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10213-2-AP targets RABEPK/p40 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag0285 Product name: Recombinant human RABEPK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 162-355 aa of BC000503 Sequence: AQPVQDTKLHVFDANTLTWSQPETLGNPPSPRHGHVMVAAGTKLFIHGGLAGDRFYDDLHCIDISDMKWQKLNPTGAAPAGCAAHSAVAMGKHVYIFGGMTPAGALDTMYQYHTEEQHWTLLKFDTLLPPGRLDHSMCIIPWPVTCASEKEGSNSLTLNHEAEKEDSADKVMSHSGDSHEESQTATLLCLVFGG |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Rab9 effector protein with kelch motifs |
| 别名 | p40, RABEPK, RAB9P40, Rab9 effector protein with kelch motifs, 40 kDa Rab9 effector protein |
| 计算分子量 | 40 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC000503 |
| 基因名称 | RABEPK/p40 |
| Gene ID (NCBI) | 10244 |
| RRID | AB_2238057 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z6M1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.
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文献和实验，还需暗室等相应实验室条件。 2.蛋白质提取方法改进 由细胞提取蛋白质常规是用含有蛋白酶抑制剂的裂解缓冲液直接裂解细胞，在收集的细胞中加入裂解液（0.5ml/107）4℃振荡60分钟，17000g，4℃离心后取上清，（裂解液配方[1]：50mM Tris-HCl，PH7.5；0.3M NaCl；1%Nonidet P40；0.1%去氧胆酸钠；0.1%SDS；1mM EDTA；10mM 焦磷酸钠；50mM 氟化钠；1mM 偏钒酸钠。临用时新鲜加入20ug/ml抑肽酶；20ug/ml亮肽酶；10
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