- ￥1350 - 3000
- Proteintech
- 10077-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, FC (Intra), ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human KPNB1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), ELISA
- 靶点：
Importin Beta
- 抗体英文名：
Importin Beta Polyclonal antibody
- 抗体名：
Importin Beta Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | A2780 cells, mouse brain tissue, rat brain tissue, HeLa cells, NIH/3T3 cells, C6 cells, HT-1080 cells, PC-12 cells |
| Positive IHC detected in | human colon cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10077-1-AP targets Importin Beta in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag0122 Product name: Recombinant human KPNB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 408-605 aa of BC003572 Sequence: QAMPTLIELMKDPSVVVRDTAAWTVGRICELLPEAAINDVYLAPLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISD |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | karyopherin (importin) beta 1 |
| 别名 | KPNB1, Importin-90, Importin subunit beta-1, Importin subunit beta 1, Importin 90 |
| 计算分子量 | 97 kDa |
| 观测分子量 | 90-97 kDa |
| GenBank蛋白编号 | BC003572 |
| 基因名称 | KPNB1 |
| Gene ID (NCBI) | 3837 |
| RRID | AB_2133977 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14974 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB,IP | Cell Res Neddylation of PTEN regulates its nuclear import and promotes tumor development.Authors - Ping Xie |
| WB | Cell Rep CGG repeats in the human FMR1 gene regulate mRNA localization and cellular stress in developing neuronsAuthors - Carissa L Sirois | |
| human | WB | Oncogene KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.Authors - Zhi-Chuan Zhu
|
| human | WB,IP | J Transl Med TC2N inhibits distant metastasis and stemness of breast cancer via blocking fatty acid synthesisAuthors - Xiang-Lin Hao |
| human | WB | Virol Sin African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.Authors - Yisha Zhuo |
| human | WB | PLoS Pathog Phospholipid scramblase 1 interacts with influenza A virus NP, impairing its nuclear import and thereby suppressing virus replication.Authors - Weiyu Luo |
2. 抗原与填料的连接 这一步是整个纯化过程中最关键的一步，如果在这一步上有什么失误，建议你从step one重新开始吧。 首先要注意一点：常见的连接方法都基于抗原上的游离氨基或者巯基与活化后的beads的连接，因此体系中不应该有其它含有氨基或巯基的化合物，包括尿素、硫脲、盐酸胍、Tris、铵根离子、Beta-巯基乙醇等，如果有这些物质在体系中，应该首先设法除去。 溴化氰活化的琼脂糖与氨基化合物反应的机理如下： 环氧氯丙烷活化的琼脂糖与氨基化合物反应
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