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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human ADD1 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
WB, IHC, IF-P, ELISA
- 靶点:
Alpha Adducin
- 抗体英文名:
Alpha Adducin Polyclonal antibody
- 抗体名:
Alpha Adducin Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | K-562 cells, mouse testis tissue |
| Positive IHC detected in | human ovary cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10791-1-AP targets Alpha Adducin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | rat, bovine |
| 免疫原 |
CatNo: Ag1245 Product name: Recombinant human ADD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC013393 Sequence: MNGDSRAAVVTSPPPTTAPHKERYFDRVDENNPEYLRERNMAPDLRQDFNMMEQKKRVSMILQSPAFCEELESMIQEQFKKGKNPTGLLALQQIADFMTTNVPNVYPAAPQGGMAALNMSLGMVTPVNDLRGSDSIAYDKGEKLLRCKLAAFYRLADLFGWSQLIYNHITTRVNSEQEHFLIVPFGLLYSEVTASSLVKINLQGDIVDRGSTNLGVNQAGFTLHSAIYAARPDVKCVVHIHTPAGAAVSAMKCGLLPISPEALSLGEVAYHDYHGILVDEEEKVLIQKNLGPKSKVLILRNHGLVSVGESVEEAFYYIHNLVVACEIQVRTLASAGGPDNLVLLNPEKYKAKSRSPGSPVGEGTGSPPKWQIGEQEFEALMRMLDNLGYRTSSSGRRQDG |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adducin 1 (alpha) |
| 别名 | ADD1, Alpha-adducin, adducin 1 (alpha), ADDA, ADD 1 |
| 计算分子量 | 81 kDa, 84 kDa, 70 kDa, 73 kDa, 56 kDa |
| 观测分子量 | 115-120 kDa |
| GenBank蛋白编号 | BC013393 |
| 基因名称 | Alpha Adducin |
| Gene ID (NCBI) | 118 |
| RRID | AB_2257952 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35611 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
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文献和实验| Species | Application | Title |
|---|---|---|
| rat | WB | Int J Mol Sci Decoding the Synaptic Proteome with Long-Term Exposure to Midazolam during Early Development.Authors - Nghi M Nguyen |
| bovine | WB | Mol Biol Rep Depletion of serum-derived exosomes aggravates heat stress-induced damage of bovine mammary epithelial cellsAuthors - Yue Wang |
accumulation by LPS, as TLR4 can use either MyD88 or TRIF. The net result of these interactions was activation of the transcription factors NF-{kappa}B and AP-1, and synthesis of TNF-{alpha} mRNA. TNF-{alpha} acts through an apparent autocrine pathway
:FITC 羊抗小鼠(绿色)稀释度 1:700 室温下孵育 1 小时。复染色:Hoechst(蓝色)细胞核染色,1:4,000 室温下孵育 1 小时, 细胞定位:路易体内含物;放大倍数:20x。 注射了 1 型小鼠α-突触核蛋白 PFFs(SPR-324)的大鼠脑免疫组织化学分析。实验物种:Sprague-Dawley 大鼠(母),在大鼠脑中有两个注射点,分别是 AP+1.6、ML+2.4、DV-4.2 和 AP-1.4、ML+0.2、DV-2.8,每个注射点注射 2 µL 1 型小鼠 α-突触核蛋白
)、Anti-IgA (alpha)】 在一些特殊的实验里,只需要检测样本里的IgG或者IgM,常规的Anti-IgG (H+L)由于与IgG的重链和轻链都有结合反应,而IgG、IgM、IgA都有保守的轻链结构域,因此不能特异性的检测某种类型的IgG或者IgM。这时候就需要针对特殊类型Ig分子的二抗。***捷备有全面的二抗现货产品并能提供最完整的二抗产品线。主要二抗品牌为全球最大的二抗产品Jackson二抗以及前沿抗体生产商EarthOx。按照标记分,有HRP、AP、生物素等标记二抗,FITC
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