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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名:
CCK-8
- 保存条件:
低温
- 供应商:
上海研卉生物
- 库存:
CK04
- 保质期:
CCK8细胞增殖与毒性检测试剂盒,CCK8
- 规格:
5000 tests
- 外 观: 粉红色液体
- 别 名: CCK-8/WST-8
假一赔十
上海研卉生物供应同仁Cell Counting Kit-8(CCK-8试剂盒)
产品规格有100 500 1000 3000T 5000T 包装
欢迎寻购。
CCK-8法与普通的MTT法相比,具有显著特点:
★灵敏度高,数据可靠,重现性好
★操作简便,省时省力
★水溶性,不需要换液,尤其适合于悬浮细胞
★无需放射性同位素和有机溶剂,对细胞毒性低
★为1瓶溶液,毋需预制,即开即用
★适合于高通量药物筛选
CCK-8用途:药物筛选、细胞增殖测定、细胞毒性测定、肿瘤药敏试验
CCK-8试剂盒在国内知名科研院所、重点大学、三甲医院被广泛应用,国际认可度高,使用CCK-8发表的中外文献达600多篇,其中截止08年底SCI影响因子IF>10分的论文有37篇,最高IF26.5分
[Heterozygosity with respect to Zfp148 causes complete loss of fetal germ cells during mouse embryogenesis,Nature Genetics 33, 172 - 176 (01 Feb 2003)]
CCK-8长效终止液-Stop Solution for CCK-8(金牌推荐)
200元/500T
参考文献:
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- 作者
- 内容
- 询问日期
文献和实验参考文献
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一、实验原理 细胞活力检测试剂盒(CCK-8) 可用于简便而准确的细胞增殖和毒性分析。 其基本原理为:该试剂中含有WST-8【化学名:2-(2-甲氧基-4-硝基苯基)-3-(4-硝基苯基)-5-(2,4-二磺酸苯)-2H-四唑单钠盐】,在电子载体1-甲氧基-5-甲基吩嗪鎓硫酸二甲酯(1-Methoxy PMS)的作用下被细胞中的脱氢酶还原为具有高度水溶性的黄色甲瓒产物(Formazan dye)。生成的甲瓒物的数量与活细胞的数量成正比。因此可利用这一特性直接进行细胞增殖和毒性分析。CCK-8法
还有就是用Invitrogen公司的CFSE细胞增殖流式检测试剂盒也可,我觉得。 qing0831 可以用流式细胞术检测,我经常做这方面的实验,可以交流一下,手机:15921609870 QQ:57325595 bigbang_0_0 cologenic assay bigbang_0_0 cologenic assay nmmxq Brdu incorporation assay
优化抗体用量、染料浓度; 4.孵育时间等参数 自动采集数据,中间无需手动操作。 实验的一般流程: 基于阻抗检测的细胞分析技术,使得实时、不间断且无需标记的细胞监测成为可能。对数据流的后续分析可以揭示细胞间互作和细胞-药物反应的动力学,可以更好地理解其机理而无需做费时费力的多次终点法实验。目前阻抗平台可用于细胞增殖、肿瘤免疫、细胞毒性及活力检测、药物筛选、信号通路(GPCR/CFTR)、细胞间相互作用 (屏障功能)、病毒学研究及细胞迁移等细胞表型研究。










