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- Miltenyi
- 130-092-654
- Miltenyi
- 2025年07月16日
- 流式
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
流式
- 宿主：
Mouse
- 抗原来源：
Mouse
- 供应商：
Miltenyi
- 库存：
大量
- 是否单克隆：
0
- 规格：
100tests
| Description |
| Clone AC128 recognizes the CD31 antigen, also known as platelet endothelial cell adhesion molecule 1 (PECAM-1), a 120–140 kDa single-chain transmembrane glycoprotein. CD31 is present on virtually all monocytes, platelets, and granulocytes. CD31 is also expressed by lymphocyte subsets. During maturation of CD4+ T cells, the expression of CD31 changes: CD4+ recent thymic emigrants (RTEs) express CD31 in contrast to central naive T cells. CD31 is highly expressed on endothelial cells and on junctions between them and is, therefore, also called ENDO-CAM. This monoclonal antibody was tested to cross-react with rhesus monkey (Macaca mulatta) cells. |
| Applications |
| The CD31 antibody can be used to identify and enumerate CD31+ cells by flow cytometry or fluorescence microscopy. |
| Clone | Isotype |
| AC128 | Mouse IgG1 |
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文献和实验（主要针对Miltenyi产品） 一、标记策略 用MACS进行磁分离，最重要的参数就是高质量的标记。对阳性细胞的标记应尽可能强，而背景要尽可能低，才能获得对磁标记的阳性细胞和未标记的阴性细胞的最好分辨。有多种不同的标记策略可供参考： 1、直接标记
Human Antibodies by Guided Selection
Guided selection is a method for humanization of pre-existing non-human (for example, mouse) antibodies, based on chain shuffling of V-genes by using phage display technology. Mouse VH and VL domains are used to guide the selection of a human
Production of Human Antibodies from Transgenic Mice
Mice transgenic for human immunoglobulin genes offer the opportunity to investigators to derive fully human antibodies using well-established hybridoma technology. Several different strains of such mice are available, and can be obtained
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