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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 保存条件:
-20℃
- 英文名:
α-Mating Factor Pheromone, Yeast
- 供应商:
亚光多肽
- 保质期:
3年
- 规格:
mg~g询价
产品信息:
序列信息:WHWLQLKPGQPMY
分子式:C82H114N20O17S1
分子量:1684 g/mol
等电点:9.3
CAS No. :59401-28-4
别名:Mating Factor α
性状:冻干粉
介绍:α-Factor Mating Pheromone, yeast 是一种十三肽,由 S. cerevisiae 的 α 细胞分泌产生,通过 Ste2p 受体起作用。
α-Factor Mating Pheromone, yeast is a tridecapeptide secreted by S. cerevisiae α cells via Ste2p receptor.
发货:冻干粉、CoA报告(附HPLC及MS图谱),顺丰包邮
应用及纯度选择建议:对于不太灵敏的筛选实验,建议使用粗品或>75%;对免疫级别,建议选用>85%;对于受体与LIGAND相互作用的研究,生物ASSAY研究,或细胞水平的研究,建议>95%;对于结构研究,建议>98%。
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文献和实验Chemotropism During Yeast Mating
yeast Saccharomyces cerevisiae undergoes chemotropic growth during mating, in which two haploid cells of opposite mating type grow toward one another. We have shown that mating pheromone gradients are essential for efficient mating in yeast
*** Make sure the agar equilibrates to 55 C to 60 C before starting the assay; otherwise it will kill the cells. 3. Distribute several paper disks (Difco, #1599-33-6) into a sterile petri dish, and spot either 5 or 15 of synthetic alpha-factor (1 - 5 mg
Setup You have yeast strains that are deficient in mating (eg Ste12 knockouts) and would like to test whether transforming them with a plasmid that contains genes that are supposed to complement the mating deficiency actually restores









