- ￥6480
- Agrisera
- 瑞典
- AS16 3199
- 2026年02月13日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
H2A.XS139p | H2A.X phosphorylated serine 139
- 规格：
50ug
| Immunogen KLH-conjugated synthetic peptide |
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Host Rabbit
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Clonality Polyclonal
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Purity Affinity purified serum in PBS with 0.05 % sodium azide
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Format Liquid
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Quantity 50 µg
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Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
|
|
Tested applications Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)
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Recommended dilution 1: 10 000 (Dot), 1: 10 000 (ELISA), 1: 1000 (WB)
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|
Expected | apparent MW
|
| 15 kDa |
名称：H2A.XS139p | H2A.X phosphorylated serine 139
货号：AS16 3199
品牌：Agrisera
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文献和实验的 相互作用，并充当效应蛋白的平台，导致下游级联事件。 磷酸化发生在所有核心组蛋白上，并且对每一个核心组蛋白都有不同的作用。组蛋白 H3 在丝氨酸 10 和 28 上的磷酸化，以及组蛋白 H2A 在 T120 上的磷酸化参与了染色质致密化以及有丝分裂过程中 染色质结构和功能的调节。这些是细胞周期和细胞生长的重要标志，在真核生物中得以保留。S139 处 H2AX 的磷酸化（产生 γH2AX）作为 DNA 损伤修复蛋白的招募点 (Lowndes et al., 2005, Pinto et al
Histone H2AX Phosphorylation: A Marker for DNA Damage
) are generated that rapidly result in the phosphorylation of histone H2A variant H2AX. Because phosphorylation of H2AX at Ser 139 (γ-H2AX) is abundant, fast, and correlates well with each DSB, it is the most sensitive marker that can be used to examine the DNA
Analysis of RB Action in DNA Damage Checkpoint Response
checkpoint response: (1) transcriptional repression of E2F-regulated genes (cyclin A reporter assay); (2) induction of cell cycle arrest (Brd-U incorporation assay); and (3) inhibition of DNA double-strand break accumulation (phosphorylated-histone H2A.X
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