- ￥1700 - 4000
- GeneTex
- 美国
- GTX127340
- 2025年07月14日
- WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm
- Rabbit
- Human, Mouse, Rat, Zebrafish
企业认证
相关产品推荐更多 >
![PSMA antibody [GCP-04]](https://img1.dxycdn.com/2022/0328/523/2712624603306500453-14.jpg!wh200)
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide surrounding phospho Ser139 of human Histone H2A.X. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Zebrafish
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX127340
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm
- 浓度：
0.16 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H2A.XS139ph (phospho Ser139)
- 抗体英文名：
Histone H2A.XS139ph (phospho Ser139) antibody
- 抗体名：
Histone H2A.XS139ph (phospho Ser139) 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Histone H2A.X (phospho Ser139) antibody detects H2AFX protein in cisplatin-treated samples by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract (untreated)
B. 30 μg NIH-3T3 whole cell lysate/extract (30μM cisplatin treatment for 24hr)
15% SDS-PAGE
Histone H2A.X (phospho Ser139) antibody (GTX127340) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Histone H2A.X (phospho Ser139) antibody detects H2AFX protein by western blot analysis.
A. 30 μg HCT116 whole cell lysate/extract (untreated for 2hr)
B. 30 μg HCT116 whole cell lysate/extract (UVB treatment 50mJ for 2hr)
C. 30 μg HCT116 whole cell lysate/extract (untreated for 6hr)
D. 30 μg HCT116 whole cell lysate/extract (UVB treatment 50mJ for 6hr)
15% SDS-PAGE
Histone H2A.X (phospho Ser139) antibody (GTX127340) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) HCT-116 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Jeong H et al., Cells 2022 (PMID:36552858)
Zhou X et al., Transl Cancer Res 2022 (PMID:36093520)
Hsueh-Tzu Shih et al., Cell Death Dis 2022 (PMID:35688824)
Shenzhen Huang et al., RSC Adv 2019 (PMID:35517706)
Tung Chao et al., Autophagy 2021 (PMID:33465003)
Vanhunsel S et al., NPJ Aging Mech Dis 2021 (PMID:34404797)
Yang JL et al., In Vivo 2021 (PMID:34410957)
Ye S et al., Cell Death Dis 2020 (PMID:32703938)
Zhao T et al., Cell Death Dis 2021 (PMID:33542214)
Cheng YC et al., In Vivo 2021 (PMID:33622876)
Lu KW et al., Anticancer Res 2020 (PMID:33288579)
Wang YY et al., ACS Omega 2020 (PMID:32258937)
Oku Y et al., FEBS Open Bio 2018 (PMID:29928579)
van Gastel J et al., Aging (Albany NY) 2019 (PMID:31794429)
Wang YY et al., Biochem Pharmacol 2020 (PMID:31733192)
Gizak A et al., Cell Death Dis 2019 (PMID:31558701)
Novoa B et al., Aging Cell 2019 (PMID:31348603)
Lan Y et al., Br J Dermatol 2019 (PMID:31380578)
Li CH et al., Toxins (Basel) 2019 (PMID:31072027)
Shenzhen Huang et al., RSC Adv. 2019 9()
Wang YY et al., Oral Dis 2019 (PMID:30620118)
Huang AC et al., Anticancer Res 2019 (PMID:30952724)
Tu HJ et al., Clin Epigenetics 2018 (PMID:30594243)
Sakamoto A et al., Cancer Sci 2019 (PMID:30426593)
Chien JY et al., Cell Death Discov 2016 (PMID:27551500)
Chen CC et al., Cell Death Dis 2018 (PMID:29749376)
Ratnadiwakara M et al., Elife 2018 (PMID:29741478)
Oku Y. et al., FEBS openbio 2018 (Epub)
Ma N et al., bioRxiv 2018 (Epub)
Calo E et al., Nature 2018 (PMID:29364875)
Tsao N et al., Cell Cycle 2015 (PMID:25659034)
Koo GB et al., Sci Rep 2017 (PMID:28611389)
Tsai CH et al., Life Sci 2016 (PMID:28007499)
Wei RJ et al., Toxicol Appl Pharmacol 2016 (PMID:27678524)
Liu MK et al., Int J Mol Sci 2016 (PMID:27314325)
Tsao N et al., Biochem J 2016 (PMID:26945015)
Yao-Yu H et al., Oncotarget 2016 (PMID:27036028)
Percival SM et al., Dis Model Mech 2015 (PMID:26044958)
May EW et al., Pharmacol Res 2014 (PMID:25242635)
Histone H2AX Phosphorylation: A Marker for DNA Damage
) are generated that rapidly result in the phosphorylation of histone H2A variant H2AX. Because phosphorylation of H2AX at Ser 139 (γ-H2AX) is abundant, fast, and correlates well with each DSB, it is the most sensitive marker that can be used to examine the DNA
组蛋白是和染色体相联的最主要的蛋白质。它的作用是和染色体中的 DNA 的负电荷结合。组蛋白是比较小的碱性蛋白；在细胞正常 pH 值时，组蛋白带有正电荷，这样它们就可以和 DNA 结合，这个正电荷主要存在于碱性氨基酸 Lys 和 Arg 的 -NH3 上。其实，组蛋白约含 25% 的 Arg 和 Lys 。比其他蛋白的 Arg ， Lys 的含量都多。和真核 DNA 结合的有 5 种类型的组蛋白： H1 ， H2A ， H2B ， H3
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
技术资料暂无技术资料 索取技术资料
