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上海觅拓生物
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文献和实验Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
on mouse tissue are of IgG1 isotype. Detailed explanation of this method can be found in: Cattoretti G, Qing Fei. Application of the Antigen Retrieval Technique in experimental Pathology: from human to mouse
Rapid Isotyping of Mouse Monoclonal Antibodies
Determination of class and subclass of monoclonal antibodies (MAbs) is important because several immunochemical and functional properties of antibodies depend on the isotype. Knowledge of the light-chain isotype (κ for 95% or λ
Immunofluorescent Staining of Mouse and Rat Leukocytes
-up, please see description in "Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis". For reducing FcgII/IIIR-mediated antibody binding (or binding of SAv-PE or SAv-Cy-Chrome) which could contribute to background, the use of anti-mouse CD32/CD
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