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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海觅拓生物
- 规格:
50 ug
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文献和实验Dual- and Triple-Color Fluorospot
labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml
50T/100T 800/1200 L0031 Mouse anti-Human CD11a mAb,PE 50T/100T 1000/1500 L0032 Mouse anti-Human CD11b mAb,FITC 50T/100T 800/1200
Immunohistochemisty-Fluorescence Protocol
the cells twice by centrifugation in Wash Buffer-Saponin (400 x g for 5 minutes). Incubate cells for 30 minutes at room temperature with 15 µL of a fluorochrome-labeled secondary antibody (either FITC-labeled anti-mouse IgG1, IgG2A or IgG2B diluted
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