重组人碱性成纤维细胞生长因子 hFGF-basic

FGF basic (FGF-2, HBGF-2) is one of at least 22 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation. Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Human and mouse mature FGF basic shares 96% sequence identity. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity. Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members. FGF basic modulates normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration.

·Description
Recombinant Human FGF-basic produced in E. coli is a non-glycosylated polypeptide chain containing 157
amino acids and having a molecular mass of 17kDa.

·Quality Control
Biological activity: The ED50 as determined by the dose-dependant proliferation of BALB/3T3 cells was found
to be less than 0.5 ng/ml, corresponding to a Specific Activity of 2.0 x 106 IU/mg.

Purity: Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found
to be Gly-Thr-Met-Ala-Ala.

Endotoxin: Less than 0.1ng/μg (1.0EU/μg) of FGF-basic as determined by LAL test.

·Formulation
Human FGF-basic was lyophilized from 1mg/ml solution after extensive dialysis against 20 mM phosphate
buffer, pH 7.2, 50 mM Na2SO4, 0.2 mM DTT and 0.2 mM EDTA.

·Reconstitution
It is recommended to reconstitute the lyophilized rhFGF-basic in sterile PBS not less than 100μg/ml, which can then be further diluted to other solutions.

·Storage
Lyophilized rhFGF-basic although stable at room temperature for 3 weeks, should be stored desiccated below -18°C, which is stable for up to twelve months from date of receipt. Upon reconstitution rhFGF-basic should be stored at 4°C between 2-7 days and aliquots of reconstituted samples with 0.1% HSA or BSA are stable up to 3 months below -18°C for future use.

Please avoid freeze-thaw cycles.