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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic peptide of human ACTN4
- 亚型:
IgG
- 保存条件:
Store at -20°C. Avoid freeze / thaw cycles.
- 适应物种:
Human Mouse Rat
- 目录编号:
K107010P
- 库存:
99
- 供应商:
北京索莱宝科技有限公司
- 应用范围:
WB IHC
- 靶点:
ACTN4
- 抗体名:
ACTININ-4,FSGS,FSGS1,alpha-actinin-4
- 规格:
50ul/100ul
| 规格: | 50ul | 产品价格: | ¥960.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1600.0 |
| 英文名称 | Anti-ACTN4 Polyclonal Antibody |
| 宿主 | Rabbit |
| 别名 | ACTININ-4,FSGS,FSGS1,alpha-actinin-4 |
| 稀释比例 | WB 1:8000-12000. IHC 1:100-300. |
| 蛋白分子量 | 105/80/60kDa |
| Gene ID | 81 |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles. |
| 储存液 | Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. |
| 纯化方法 | Affinity purification |
| 亚型 | IgG |
| 免疫原 | A synthetic peptide of human ACTN4 |
| 性状 | 液体 |
| 亚细胞定位 | Cytoplasm Nucleus |
| Swiss Prot | O43707 |
| 背景资料 | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. |
| Public Immunogen Range | 75-125/911aa |
| 克隆类型 | Polyclonal Antibody |
| 交叉反应 | Human Rat Mouse |
| 应用 | WB IHC |
| 规格 | 50ul 100ul |
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
实验图
Western blot analysis with ACTN4 antibody diluted at 1:10000;Lane:A375,NIH3T3
Immunohistochemistry?of?paraffin-embedded?Human pancreatic cancer?with?ACTN4 antibody diluted at 1:200
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文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇:make an Antibody against a Peptides 下一篇:Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
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