- ￥960 - 1600
- Solarbio
- 北京
- K101310P
- 2025年07月16日
- WB
- Rabbit
- Human Mouse Rat
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide of human PCNA
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid freeze / thaw cycles.
- 克隆性：
单克隆
- 适应物种：
Human Mouse Rat
- 目录编号：
K101310P
- 库存：
99
- 供应商：
北京索莱宝科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB
- 靶点：
PCNA
- 抗体名：
ATLD2;cb16;Cyclin;HGCN872;MGC8367
- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥960.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1600.0 |
| 英文名称 | Anti-PCNA Polyclonal Antibody |
| 中文名称 | 增殖细胞核抗原抗体 |
| 别名 | Proliferation Marker, Cyclin, DNA polymerase delta auxiliary protein, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, Pcna/cyclin, PCNAR, Polymerase delta accessory protein, Proliferating Cell Nuclear Antigen, PCNA_HUMAN |
| 背景资料 | Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. |
| Swiss Prot | P12004 |
| Gene ID | 5111 |
| 亚细胞定位 | Nucleus |
| 宿主 | Rabbit |
| 克隆类型 | Polyclonal Antibody |
| 亚型 | IgG |
| 蛋白分子量 | 29kDa |
| 稀释比例 | WB 1:500-2000. |
| 免疫原 | A synthetic peptide of human PCNA |
| Public Immunogen Range | 150-200/261aa |
| 纯化方法 | Affinity purification |
| 储存液 | Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles. |
| 性状 | 液体 |
| 交叉反应 | Human Rat Mouse |
| 应用 | WB |
| 规格 | 50ul 100ul |
| 单位 | 支 |
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示：
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图
Western blot analysis with PCNA antibody diluted at 1:2000;Lane: HepG2,Jurkat,MCF7,HeLa,293T,Raw 264.7,NIH/3T3.
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- 作者
- 内容
- 询问日期
文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇：make an Antibody against a Peptides 下一篇：Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
a simple ELISA method to detect human anti-antibody response.
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