- ￥960 - 1600
- Solarbio
- 北京
- K001460M
- 2025年09月30日
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified recombinant full length of human Leptin protein expressed in E.coli.
- 亚型：
IgG2a
- 形态：
液体
- 保存条件：
Store at -20°C. Avoid freeze / thaw cycles.
- 克隆性：
单克隆
- 适应物种：
Human
- 目录编号：
K001460M
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- 宿主：
Mouse
- 靶点：
Leptin
- 抗体英文名：
Anti-Leptin Monoclonal Antibody
- 抗体名：
LEPD;OB;OBS
- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥960.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1600.0 |
产品简介
备注： 以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。 These protocols are for reference only. Solarbio does not independently validate these methods. 温馨提示： 使用前请离心。如有问题请及时联系我们。 Quick spin before use. If you have any questions, please contact us. |
Mouse | ||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 英文名称 | Anti-LEP Monoclonal Antibody | ||||||||||||||||||||||||||||||||||||||||||
| 别名 | LEPD;OB;OBS;leptin | ||||||||||||||||||||||||||||||||||||||||||
| 稀释比例 | WB 1:1000. | ||||||||||||||||||||||||||||||||||||||||||
| 蛋白分子量 | 19kDa | ||||||||||||||||||||||||||||||||||||||||||
| Gene ID | 3952 | ||||||||||||||||||||||||||||||||||||||||||
| 保存 | Store at -20°C. Avoid freeze / thaw cycles. | ||||||||||||||||||||||||||||||||||||||||||
| 储存液 | Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | ||||||||||||||||||||||||||||||||||||||||||
| 纯化方法 | Affinity purification | ||||||||||||||||||||||||||||||||||||||||||
| 亚型 | IgG2a | ||||||||||||||||||||||||||||||||||||||||||
| 免疫原 | Purified recombinant full length of human LEP protein expressed in E.coli. | ||||||||||||||||||||||||||||||||||||||||||
| 性状 | 液体 | ||||||||||||||||||||||||||||||||||||||||||
| 亚细胞定位 | Secreted | ||||||||||||||||||||||||||||||||||||||||||
| Swiss Prot | P41159 | ||||||||||||||||||||||||||||||||||||||||||
| 克隆号 | 5C11-G6-B12 | ||||||||||||||||||||||||||||||||||||||||||
| 克隆类型 | Monoclonal Antibody | ||||||||||||||||||||||||||||||||||||||||||
| 背景资料 | This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. | ||||||||||||||||||||||||||||||||||||||||||
| 应用 | ELISA | ||||||||||||||||||||||||||||||||||||||||||
| 交叉反应 | Human | ||||||||||||||||||||||||||||||||||||||||||
| 规格 | 50ul 100ul |
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文献和实验指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
a simple ELISA method to detect human anti-antibody response.
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