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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant human FAK protein fragments expressed in E.coli.
- 亚型:
IgG1
- 形态:
液体
- 保存条件:
Store at -20°C. Avoid freeze / thaw cycles.
- 克隆性:
单克隆
- 标记物:
详询
- 适应物种:
Human
- 保质期:
详询
- 抗原来源:
详询
- 目录编号:
K000112M
- 级别:
详询
- 库存:
999
- 供应商:
北京索莱宝科技有限公司
- 宿主:
Mouse
- 应用范围:
IP ; ICC ; IF ; WB
- 浓度:
详询
- 靶点:
FAK
- 抗体英文名:
Anti-FAK Monoclonal Antibody
- 抗体名:
FADK;FAK;FAK1;FRNK;p125FAK;pp125FAK;PPP1R71
- 规格:
50ul/100ul
| 规格: | 50ul | 产品价格: | ¥960.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1600.0 |
产品简介
|
英文名称
|
Anti-PTK2 Monoclonal Antibody
|
|
中文名称
|
粘附斑激酶抗体
|
|
靶标
|
PTK2
|
|
别名
|
PTK2;FADK;FAK;FAK1;FRNK;PPP1R71;p125FAK;pp125FAK;focal adhesion kinase 1
|
|
背景资料
|
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined.
|
|
Swiss Prot
|
Q05397
|
|
Gene ID
|
5747
|
|
亚细胞定位
|
Cell junction Cell membrane Cytoplasm Cytoplasmic side Nucleus Peripheral membrane protein cell cortex centrosome cytoskeleton focal adhesion microtubule organizing center
|
|
宿主
|
Mouse
|
|
克隆类型
|
Monoclonal Antibody
|
|
亚型
|
IgG1
|
|
克隆号
|
3D8-H11-E12
|
|
交叉反应
|
Human
|
|
蛋白分子量
|
40/49/63/99/121/114/119kDa
|
|
应用
|
IP ; ICC ; IF ; WB
|
|
稀释比例
|
WB 1:500-1000. IF 1:50-200. IP 1:20. ICC 1:50-200.
|
|
免疫原
|
Recombinant protein of human PTK2
|
|
纯化方法
|
Affinity purification
|
|
储存液
|
Buffer: PBS with 0.02% sodium azide, 0.5% BSA, 50% glycerol, pH7.3.
|
|
保存
|
Store at -20℃. Avoid freeze / thaw cycles.
|
|
规格
|
100ul ; 50ul
|
|
单位
|
支
|
These protocols are for reference only. Solarbio does not independently validate these methods.
Quick spin before use. If you have any questions, please contact us.
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- 作者
- 内容
- 询问日期
文献和实验指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma,在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应,对应于抗原之不同部分可制成各种不同的抗体复合体。然而,因为一个产生抗体的细胞,只能产生一种抗体,所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种,可形成边产生抗体边增殖的 hybridoma,将每个杂种细胞进行克隆(无性繁殖系)培养,则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成,这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
a simple ELISA method to detect human anti-antibody response.











