相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant human P38 MAPK Pprotein fragments expressed in E.coli.
- 亚型:
IgG1
- 形态:
液体
- 保存条件:
Store at -20°C. Avoid freeze / thaw cycles.
- 克隆性:
单克隆
- 标记物:
详询
- 适应物种:
Human Mouse Rat Monkey
- 保质期:
详询
- 抗原来源:
详询
- 目录编号:
K000093M
- 级别:
详询
- 库存:
999
- 供应商:
北京索莱宝科技有限公司
- 宿主:
Mouse
- 应用范围:
WB
- 浓度:
详询
- 靶点:
p38 MAPK
- 抗体英文名:
Anti-p38 MAPK Monoclonal Antibody
- 抗体名:
CSBP;CSBP1;CSBP2;CSPB1;EXIP;Mxi2;p38;p38ALPHA;PRKM14;PRKM15;RK;SAPK2A
- 规格:
50ul/100ul
| 规格: | 50ul | 产品价格: | ¥960.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1600.0 |
|
Anti-MAPK14 Monoclonal Antibody
|
|
|
中文名称
|
丝裂原活化蛋白激酶14抗体
|
|
靶标
|
MAPK14
|
|
别名
|
CSBP;CSBP1;CSBP2;CSPB1;EXIP;Mxi2;p38;p38ALPHA;PRKM14;PRKM15;RK;SAPK2A
|
|
背景资料
|
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
|
|
Swiss Prot
|
Q16539
|
|
Gene ID
|
1432
|
|
亚细胞定位
|
Cytoplasm Nucleus
|
|
宿主
|
Mouse
|
|
克隆类型
|
Monoclonal Antibody
|
|
亚型
|
IgG1
|
|
克隆号
|
5A1-C5-F11
|
|
交叉反应
|
Mouse ; Rat ; Monkey ; Human
|
|
蛋白分子量
|
40kDa
|
|
应用
|
WB
|
|
稀释比例
|
WB 1:500-1000.
|
|
免疫原
|
Recombinant protein of human MAPK14
|
|
纯化方法
|
Affinity purification
|
|
储存液
|
Buffer: PBS with 0.02% sodium azide, 0.5% BSA, 50% glycerol, pH7.3.
|
|
保存
|
Store at -20℃. Avoid freeze / thaw cycles.
|
|
规格
|
100ul ; 50ul
|
|
单位
|
支
|
These protocols are for reference only. Solarbio does not independently validate these methods.
Quick spin before use. If you have any questions, please contact us.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma,在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应,对应于抗原之不同部分可制成各种不同的抗体复合体。然而,因为一个产生抗体的细胞,只能产生一种抗体,所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种,可形成边产生抗体边增殖的 hybridoma,将每个杂种细胞进行克隆(无性繁殖系)培养,则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成,这样得到的就是单克隆抗体。此抗体在测定
【求助】请问如何检测RAS、MAPK、p38、p21 ?参与者:liqunfeng请问如何检测RAS、MAPK、p38、p21??谁帮帮我啊参与者:xiaolx你在网上搜搜别人的实验中怎么检测的,不就知道了。p38,我师姐做过免疫组化。参与者:zhangyuelin1999检测信号通路现在用的比较多的方法应该是Western blot,它是测细胞中的蛋白含量(半定量)的最好方法了,免疫组化检测是定性的!如果时间充裕的话,你可以两种方法都可以做的。RAS:Ras蛋白是细胞内的一种小分子GTP结合
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer











