- ￥960 - 1600
- Solarbio
- 北京
- K000092M
- 2025年07月12日
- WB
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified recombinant Placental alkaline phosphatase (PLAP) protein fragments expressed in E.coli.
- 亚型：
IgG1
- 形态：
液体
- 保存条件：
Store at -20°C. Avoid freeze / thaw cycles.
- 克隆性：
单克隆
- 标记物：
详询
- 适应物种：
Human
- 保质期：
详询
- 抗原来源：
详询
- 目录编号：
K000092M
- 级别：
详询
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- 宿主：
Mouse
- 应用范围：
WB
- 浓度：
详询
- 靶点：
Placental alkaline phosphatase (PLAP)
- 抗体英文名：
Anti-Placental alkaline phosphatase (PLAP) Monoclonal Antibody
- 抗体名：
ALP;PALP;PLAP;PLAP-1
- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥960.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1600.0 |
产品简介
|
英文名称
|
Anti-ALPP Monoclonal Antibody
|
|
中文名称
|
碱性磷酸酶(胎盘型)抗体
|
|
靶标
|
ALPP
|
|
别名
|
Alkaline phosphatase, Alkaline phosphatase placental, Alkaline phosphatase placental type, Alkaline phosphatase Regan isozyme, ALP, Alp1, ALPP, FLJ61142, Germ-cell alkaline phosphatase, nagao isozyme,
|
|
背景资料
|
The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.
|
|
Swiss Prot
|
P05187
|
|
Gene ID
|
250
|
|
亚细胞定位
|
Cell membrane GPI-anchor Lipid-anchor
|
|
宿主
|
Mouse
|
|
克隆类型
|
Monoclonal Antibody
|
|
亚型
|
IgG1
|
|
克隆号
|
8A11-C11-C9
|
|
交叉反应
|
Human
|
|
蛋白分子量
|
70kDa
|
|
应用
|
WB
|
|
稀释比例
|
WB 1:500-1000.
|
|
免疫原
|
Recombinant protein of ALPP
|
|
纯化方法
|
Affinity purification
|
|
储存液
|
Buffer: PBS with 0.02% sodium azide, 0.5% BSA, 50% glycerol, pH7.3.
|
|
保存
|
Store at -20℃. Avoid freeze / thaw cycles.
|
|
规格
|
100ul ; 50ul
|
|
单位
|
支
|
These protocols are for reference only. Solarbio does not independently validate these methods.
Quick spin before use. If you have any questions, please contact us.
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文献和实验Human Placental Alkaline Phosphatase Stain
The following protocol is for detection of human placental alkaline phosphatase (AP) in cultured cells. Human placental AP is heat stable, unlike other AP proteins typically expressed by cells. A few cell types (HeLa cells, for example
Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48)
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
phosphatase gene, PLAP (Kam et al 1985), is perhaps the most useful as it is very heat stable and is resistant to some chemical inhibitors that are active on other endogenous alkaline phosphatases. PLAP has been used as a histochemical reporter fairly recently
