- ￥960 - 1600
- Solarbio
- 北京
- K000007M
- 2025年07月07日
- IP ; WB
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified recombinant Dynactin 1 protein fragments expressed in E.coli
- 亚型：
IgG2b
- 形态：
液体
- 保存条件：
Store at -20°C. Avoid freeze / thaw cycles.
- 克隆性：
单克隆
- 标记物：
详询
- 适应物种：
Human
- 保质期：
详询
- 抗原来源：
详询
- 目录编号：
K000007M
- 级别：
详询
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- 宿主：
Mouse
- 应用范围：
IP ; WB
- 浓度：
详询
- 靶点：
Dynactin 1(N-term)
- 抗体英文名：
Anti-Dynactin 1(N-term) Monoclonal Antibody
- 抗体名：
DAP-150;DP-150;P135
- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥960.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1600.0 |
| 宿主 | Mouse |
|---|---|
| 英文名称 | Anti-DCTN1(N-term) Monoclonal Antibody |
| 别名 | DAP-150;DP-150;P135;Dynactin 1 |
| 稀释比例 | WB 1:500. |
| 蛋白分子量 | 127/137/139/141/142kDa |
| Gene ID | 1639 |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles. |
| 储存液 | Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| 纯化方法 | Affinity purification |
| 亚型 | IgG2b |
| 免疫原 | Purified recombinant DCTN1 protein fragments expressed in E.coli |
| 性状 | 液体 |
| 亚细胞定位 | Cytoplasm cytoskeleton |
| Swiss Prot | Q14203 |
| 克隆号 | 3D5-C6-D5 |
| 克隆类型 | Monoclonal Antibody |
| 背景资料 | This gene encodes the largest subunit of dynactin,a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150kD.Dynactin binds to both microtubules and cytoplasmic dynein.Dynactin is involved in a diverse array of cellular functions,including ER-to-Golgi transport,the centripetal movement of lysosomes and endosomes,spindle formation,chromosome movement,nuclear positioning, and axonogenesis.This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus.Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA).[provided by RefSeq, Oct 2008] |
| 应用 | WB IP |
| 交叉反应 | Human |
| 规格 | 50ul 100ul |
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文献和实验In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
monoclonal antibody production fusion
, 50 ml + NaOH, 1N, 100 µl-> heat to 37ѓC, add ddH2 O up to 250 ml 上一篇：Antibody Conjugation Protocol 下一篇：Expanding Hybridoma Clones
