FRATAXIN ANTIBODY WITH CONTROL

稳定表达具有生物活性的人重组对氧磷酶

of paraoxonase-1 activity in lysate The bioactivity of paraoxonase-1 present in the cell lysate was demonstrated by its ability to hydrolyse 4 mM phenyl acetate in 20 mM Tris HCl buffer pH 8.0, containing 1 mM CaCl2. As a control 10 mM EDTA was added

Far-Western Blotting

any bands resulting from endogenous biotin in the sample or nonspecific binding of the labeled streptavidin. When a fusion tag is used with a corresponding antibody, it is critical to probe one of the control membranes with the labeled antibody

Transcription, Translation of S35-Radiolabelled Protein and Binding to GST-Fused Proteins or Antibodies (IP)

reticulocyte lysate. S35 methionine is DuPont translation grade (5mCi/mmol) Heat RNA 60°C 5 min, ice and prepare reaction cocktail. The lysate can be refrozen once. Only prepare a cocktail with the amount required. For example