- $615
- LSBio
- To be determined
- LS-F2195
- 美国
- 2025年07月14日
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- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Mouse, Human, Rat
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
To be determined
- 供应商：
康朗生物
- 规格：
96T
Mouse/Human/Rat NUDC ELISA Kit (Cell-Based ELISA) - LS-F2195
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Product Description
LS-F2195 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human NUDC in samples of Adherent Cell Cultures. It is based upon a Cell-Based assay principle.Specifications
TypeCell-Based ELISA (enzyme-linked immunosorbent assay) kit
Target
NUDC
Reactivity
Mouse, Human, Rat
Specificity
NudC
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Qualitative
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Cell Culture Microplate
- Blocking Buffer
- Detection Antibodies
- HRP-Conjugate
- Substrate
- Stop Solution
- Crystal Violet Solution
- SDS Solution
- Adhesive Plate Sealers
Protocol
- Seed cells into wells and incubate overnight at 37°C, 5% CO2.
- Apply desired treatment conditions.
- Add 100 µl of Fixing Solution and incubate 20 minutes at room temperature.
- Add 100 µl of Quenching Buffer and incubate 20 minutes at room temperature.
- Add 200 µl of Blocking Buffer and incubate for 1 hour at room temperature.
- Add 50 µl of 1x primary antibodies and incubate overnight at 4°C. Add 50 µl of HRP-conjugated secondary antibodies and incubate for 1.5 hours at room temperature.
- Add 50 µl of Ready-to-Use Substrate and incubate for 30 minutes at room temperature.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About NUDC
Q9Y266 NM_006600 NP_006591.1
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Cell-Based ELISA Platform Overview
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