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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
保存在常温(RT),保持干燥,3年
- 保质期:
保存在常温(RT),保持干燥,3年
- 英文名:
Yeast Nitrogen Base Without Amino acids
- 库存:
999
- 供应商:
北京索莱宝科技有限公司
- 规格:
100g/500g/1kg/5kg
| 规格: | 100g | 产品价格: | ¥400.0 |
|---|---|---|---|
| 规格: | 500g | 产品价格: | ¥1760.0 |
| 规格: | 1kg | 产品价格: | ¥3000.0 |
| 规格: | 5kg | 产品价格: | ¥12000.0 |
使用方法:
称量6.7g,加入5g葡萄糖于1000mL蒸馏水中,充分溶解后,过滤除菌备用;(添加量可根据需求按其工艺调整)。
YNB培养基成分:(g/L)
硫酸铵 5.0g 肌醇 2000.0μg
硫酸铜 40.0μg 烟酸 400.0μg
磷酸二氢钾 1.0g 硼酸 500.0μg
碘化钾 100.0μg 对氨-基苯甲酸 200.0μg
生物素 2.0μg 硫酸镁 0.5g
FeCL 200.0μg 盐酸吡哆醇 400.0μg
泛酸钙 2.0μg 氯化钠 0.1g
硫酸锰 400.0μg 核黄素 200.0μg
叶酸 2.0μg 氯化钙 0.1g
钼酸钠 200.0μg 盐酸硫胺素 400.0μg
硫酸锌 400.0μg
备注:产品信息可能会有优化升级。请以实际标签信息为准。
常见问题
问:过滤除菌的时候用有机系滤膜还是水系滤膜?
答:您好,使用水系滤膜进行过滤除菌即可。
问:请问无氨基酵母氮源(YNB)/YNB培养基里面的配方含有5g的mmonium Sulfate,是每多少g混合物里面的含量?
答:您好,每升YNB培养基里含有Ammonium Sulfate的质量是5g。
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成份: L-氨基酸 0.5克 蛋白胨 0.5克 牛肉膏 0.5克 葡萄糖 0.05克 吡多醛 0.5克 水 100毫升 2%溴甲酚紫乙醇液 0.5毫升 2%甲酚红 0.25毫升 制法:除指示剂及氨基酸外,溶解,PH6.0,然后加入氨基酸(必要时重新调PH)及指示剂,混匀分装1毫升/管加10毫米厚石蜡油,高压10--15磅10--15分钟灭菌备用. 注意: 1 使用DL型AA时量加倍. 2 最好同时接种空白管(即不含
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问:最近在做酵母双杂交,有些营养缺陷培养基要单独加氨基酸,不知道这些氨基酸配成母液以后能不能高温高压灭菌啊?请各位高手指教,谢谢!答:一般来说,在配制培养基过程中,在高温高压过程中易配破坏的成分如葡萄糖,氨基酸,抗生素等,多采用0.22um的滤膜过滤除菌。所以,建议楼主最好,过滤除菌后单独加入。问:基酸不能高压灭菌吗??可是在测定蛋白质中氨基酸含量的时候,酸解的条件可是110℃浓盐酸的条件下啊,这个反应里的氨基酸可是可以跑HPLC的,而且氨基酸也基本不存在空间构像问题。我在有些文献中见过氨基










