IC0920 虫草素 代谢酶/蛋白酶 索莱宝

使用本产品的案例（仅供参考）

In Vitro

Arabidopsis（14 day old，Col-0, dcp5-1 and ssf-2 seedlings；1 mM Cordycepin）

Briefly, 14-day-old Col-0, dcp5-1 and ssf-2 seedlings were incubated in 3 mL of incubation buffer (1 mM Tris–HCl pH 7.5, 1 mM NaCl, 1 mM KCl, 15 mM sucrose) with rotation at 75 rpm in 12-well plates for 15 min. The incubation solution was then replaced with 3 mL of fresh buffer containing 1 mM cordycepin (Solarbio, Beijing, China, cat: 70-03-0).   Samples incubated at 22 °C were harvested at different time points (0.5, 1, 2, 4, 6, and 12 h), frozen in liquid nitrogen, and subjected to total RNA isolation and RT-qPCR analysis as de_x005f scribed above.

来源文献：Wang W, Wang C, Wang Y, Ma J, Wang T, Tao Z, Liu P, Li S, Hu Y, Gu A, Wang H, Qiu C, Li P. The P-body component DECAPPING5 and the floral repressor SISTER OF FCA regulate FLOWERING LOCUS C transcription in Arabidopsis.   Plant Cell. 2023 May 23:koad151.   doi: 10.1093/plcell/koad151.   Epub ahead of print.   PMID: 37220754.

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标题:A double-stranded RNA binding protein enhances drought resistance via protein phase separation in rice

成员:Wang Huaijun, Ye Tiantian, Guo Zilong, Yao Yilong, Tu Haifu, Wang Pengfei, Zhang Yu, Wang Yao, Li Xiaokai, Li Bingchen, Xiong Haiyan, Lai Xuelei, Xiong Lizhong

论文因子:14.7 发表期刊:Nature Communications pmid:38514621

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标题:ALBA proteins confer thermotolerance through stabilizing HSF messenger RNAs in cytoplasmic granules

成员:Tong Jinjin, Ren Zhitong, Sun Linhua, Zhou Sixian, Yuan Wei, Hui Yufan, Ci Dong, Wang Wei, Fan Liu-Min, Wu Zhe, Qian Weiqiang

论文因子:17.352 发表期刊:Nature Plants pmid:35817823

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标题:The P-body component DECAPPING5 and the floral repressor SISTER OF FCA regulate FLOWERING LOCUS C transcription in Arabidopsis

成员:Wang Wanyi, Wang Chuanhong, Wang Yunhe, Ma Jing, Wang Tengyue, Tao Zhen, Liu Peipei, Li Shuai, Hu Yuanyuan, Gu Aiju, Wang Hui, Qiu Chunhong, Li Peijin

论文因子:12.085 发表期刊:PLANT CELL pmid:37220754

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标题:The P-body component DECAPPING5 and the floral repressor SISTER OF FCA regulate FLOWERING LOCUS C transcription in Arabidopsis

成员:Wang W; Wang C; Wang Y; Ma J; Wang T; Tao Z; Liu P

论文因子:11.6 发表期刊:Plant Cell pmid:37220754

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标题:ALBA proteins confer thermotolerance through stabilizing HSF messenger RNAs in cytoplasmic granules

成员:Tong J. Ren Z. Sun L. Zhou S. Yuan W. Hui Y. Ci D.

论文因子:7.11 发表期刊:Nat Plants pmid:35817823

基本信息
CAS	No.73-03-0
中文名称	虫草素
英文名称	Cordycepin
别名	3-Deoxyadenosine
分子式	C10H13N5O3
分子量	251.24
溶解性	Soluble in DMSO ≥5mg/mL;Soluble in Water ≥3mg/mL
纯度	HPLC≥98%
外观(性状)	White to off-white Solid
储存条件	Powder:-20℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
EC	EINECS 200-791-4
MDL	MFCD00037998
SMILES	OC[C@@H]1C[C@H]([C@H](N2C=NC3=C2N=CN=C3N)O1)O
InChIKey	OFEZSBMBBKLLBJ-BAJZRUMYSA-N
InChI	InChI=1S/C10H13N5O3/c11-8-7-9(13-3-12-8)15(4-14-7)10-6(17)1-5(2-16)18-10/h3-6,10,16-17H,1-2H2,(H2,11,12,13)/t5-,6+,10+/m0/s1
PubChem CID	6303
靶点	MMP
通路	Metabolic Enzyme&Protease
背景说明	Cordycepin是一种核苷衍生物,可以抑制 IL-1β 诱导的 MMP-1 和 MMP-3 表达。还可以通过抑制细菌的腺苷激酶杀死MTB。
生物活性	Cordycepin是IL-1β诱导的趋化因子产生和MMP表达的有效抑制剂，并且强烈阻断RASF中的p38/JNK/AP-1信号传导途径。[1]
In Vitro	Cordycepin 是从冬虫夏草中分离的一种核苷衍生物，在类风湿性关节炎滑膜成纤维细胞(RASF)中，抑制 IL-1β 诱导的 MMP-1 和 MMP-3 表达，这种作用存在剂量依赖性。使用MTT测定评估虫草素对RASF的细胞毒性的影响。用虫草素(50μM或100μM)处理RASF 24小时不会引起细胞活力的任何显著变化。然而，当细胞与100μMB冬虫夏草孵育48小时后，细胞活力略有下降[1]。
细胞实验	用各种浓度的Cordycepin(50μM或100μM)处理RASF(2×10 4个细胞/孔)。温育1小时，12小时和24小时后，用PBS洗涤细胞两次，向每个孔中加入MTT(0.5mg/mL PBS)并在37℃下孵育30分钟。通过添加DMSO(100μL/孔)溶解形成的Formazan晶体，并使用酶标仪[1]在570nm处读取吸光度。
数据来源文献	[1]. Noh EM， et al. Cordycepin inhibits IL-1beta-induced MMP-1 and MMP-3 expression in rheumatoid arthritis synovial fibroblasts. Rheumatology (Oxford). 2009 Jan;48(1):45-8
单位	瓶