槲皮素 分析对照品

本品为分析标准品。

使用本产品的案例（仅供参考）

LC-MS

The content of flavonol glycosides in G. biloba was determinedby HPLC-mass spectrometry (HPLC-MS) using an C18 column (150×12 mm,5 µm). The solvents used for separation were acetonitrile (solvent A) andwater containing 0.1% methanol (solvent B). Gradient elution was performed as follows: 0–12 min(20% A+80% B), 12–13 min (60% A + 40% B), 13–16 min (100% A), and 16–20 min (20% A +80% B).The following settings were applied: flow rate, 0.3 mL/min;column temperature, 35℃;injectionvolume, 10 µL;UV detector, 370 nm. In addition, HPLCI-MS adopts a negative ion mode, and massacquisition is the MRM mode.

来源文献：Xu N, Liu S, Lu Z, Pang S, Wang L, Wang L, Li W. Gene Expression Profiles and Flavonoid Accumulation during Salt Stress in Ginkgo biloba Seedlings. Plants (Basel). 2020 Sep 8;9(9):1162. doi: 10.3390/plants9091162. PMID: 32911855;PMCID: PMC7570044.

LC-ESI-MS/MS

The chromatographic conditions were as follows: BEH C18 chromatographic column (150 mm × 2.1 mm, 1.7 μm);mobile phase: A phase water, B phase acetonitrile, 0.1% (vol/vol) formic acid and 0.2 mM ammonium acetate were added t both phases. Step-stripping sequence: 0–1.0 min, 10% B;0–9.0 min, 10% B–90% B;9.0–11.0 min, 90% B–100% B;11.0–11.1 min, 100% B–10% B;11.1–13.0 min, 10% B. Column temperature: 30℃;injection volume: 2 μl;flow rate: 0.25 ml/min. Mass spectrum conditions: retention time 4.94 min−1;ion pair l: quantitative ion 433.1/271.1, collision energy −22 V;ion pair 2: quantitative ion 433.1/150.9, collision energy −42 V.

来源文献：Sun M, Li L, Wang C, Wang L, Lu D, Shen D, Wang J, Jiang C, Cheng L, Pan X, Yang A, Wang Y, Zhu X, Li B, Li Y, Zhang F. Naringenin confers defence against Phytophthora nicotianae through antimicrobial activity and induction of pathogen resistance in tobacco. Mol Plant Pathol. 2022 Dec;23(12):1737-1750. doi: 10.1111/mpp.13255. Epub 2022 Sep 12. PMID: 36094814;PMCID: PMC9644278.

UPLC-MS

Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) was performed with liquid mass spectrometer for evaluation. The determination was performed on the SB-C18 column at the flow rate of 0.3 mL/min and the column temperature was 40 ℃. The flavonoid and anthocyanin components were detected at 210 nm.

来源文献：Shi M, Ali MM, He Y, Ma S, Rizwan HM, Yang Q, Li B, Lin Z, Chen F. Flavonoids Accumulation in Fruit Peel and Expression Profiling of Related Genes in Purple (Passiflora edulis f. edulis) and Yellow (Passiflora edulis f. flavicarpa) Passion Fruits. Plants (Basel). 2021 Oct 20;10(11):2240. doi: 10.3390/plants10112240. PMID: 34834602;PMCID: PMC8620868.

HPLC

HPLC analysis was performed using HPLC equipped with a C18 250 mm × 4.6 mm, 5 µm column . The analysis was maintained at a column temperature of 30℃with an injection volume of 10 µL at a flow rate of 1.0 mL/min. The mobile phase A was methanol and mobile phase B was ultrapure water containing 0.1% (m/v) formic acid. The gradient elution was performed as follows: 0–5 min, B 95–90%, 5–30 min, B 90–80%, 30–45 min, B 80–76%, 45–60 min, B 76–61%, 60–70 min, B 61–57%, 70–80 min, B 57–53%, 80–85 min, B 53–95%, 85–5 min, B 95%. The separated phenolic components were monitored at a wavelength of 280 nm.

来源文献：Zhang X, Li Y, Li Y, Zhao J, Cheng Y, Wang Y, Guan J. Changes of Bioactive Components and Antioxidant Capacity of Pear Ferment in Simulated Gastrointestinal Digestion In Vitro. Foods. 2023 Mar 13;12(6):1211. doi: 10.3390/foods12061211. PMID: 36981138;PMCID: PMC10048753.

HPLC–MS

We used an HPLC system, which consisted of a binary solvent delivery system, an on-line degasser, an auto-sampler, a column temperature controller, and a diode array detector. Separation was conducted using a C18 column (5 µm, 150 mm × 4.6 mm), and the temperature was set to 40℃. The injection volume was 0.01 mL, and the flow rate was 1 mL/min. Gradient elution was conducted using a binary system consisting of 10 % formic acid in DW (A) and 10 % formic acid in acetonitrile (B). The utilized gradient was as follows: isocratic 1 % B for 6 min, 1% – 3% B for 4 min, 3 % – 15 % B for 20 min, 15 % – 40 % B for 20 min, 40 % – 90 % B for 5 min, 90 % B isocratic for 10 min, 90 % – 1 % B for 5 min, and 1 % B isocratic for 10 min. A re-equilibration operation followed. The UV detection wavelength was set to 280 nm.

The voltages of the sample cone and the capillary were set to 17 and 3000 V for the positive ion mode and 22 and 2000 V for the negative ion mode.

来源文献：[1]Zhang,Junhong,Tian,et al.Isolation and identification of phenolic compounds in Chinese purple yam and evaluation of antioxidant activity[J].LWT-Food Science &Technology, 2018, 96:161-165.

UHPLC

Chromatographic separation of SPLFA and SPLF was done on an Agilent 1290 UHPLC system coupled to an auto sampler, a diode array detector (DAD) and an ACQUITY UHPLC® HSS T3 column (2.1 ×100 mm, 1.8 μm;Waters, Milford, MA, USA)maintained at 30 ℃. Eluent A was 0.05% formic acid in water and eluent B was 0.05% formic acid in acetonitrile. Elution of the SPLPA compounds was achieved using the following linear gradient elution (in %B): 0 min, 5%;20 min, 60%;20.1 min, 5%;30 min, 5%, and for the SPLF compounds: 0 min, 20%, 25 min, 50%, 25.1 min, 20%, 35 min, 20%. The injection volume was 5 μL, with a flow rate of 0.3 mL/ min. All sample solutions were filtered through a 0.22 μm PTFE filter (Agilent Technologies) before analysis. The detection wavelength was 320 nm for SPLPA and 254 nm for SPLF.

来源文献：[1] Luo D , Mu T , Sun H .Profiling of phenolic acids and flavonoids in sweet potato (Ipomoea batatas L.) leaves and evaluation of their anti-oxidant and hypoglycemic activities[J].Food Bioscience, 2020, 39(44):100801.DOI:10.1016/j.fbio.2020.100801.

HPLC

HPLC analysis employed an Agilent 1260 HPLC instrument and a diode array detector (Agilent, Santa Clara, CA, USA). The chromatographic separation was achieved by a Zobax Stablebond Analytical SB-C18 column (250 × 4.6 mm, 5 μm). The mobile phases, containing a gradient of solvent A (acetic/water, 2:98, v/v) and solvent B (acetic acid/acetonitrile/water, 5:50:45, v/v) were used to elute the column. The solvent gradient was programmed from 90% to 55%A and 10% to 45%B for 50 min, 55% to 0%A and 45% to 100%B for 10 min, and 0% to 90%A and 100% to 10%B for 5 min. The flow velocity was 1.0 mL·min-1 , the column temperature was 30℃, and the injection volume was 20 μL

来源文献：Liang D, Deng H, Deng Q, Lin L, Lv X, Wang J, Wang Z, Xiong B, Zhao X, Xia H. Dynamic Changes of Phenolic Compounds and Their Associated Gene Expression Profiles Occurring during Fruit Development and Ripening of the Donghong Kiwifruit. J Agric Food Chem. 2020 Oct 14;68(41):11421-11433. doi: 10.1021/acs.jafc.0c04438. Epub 2020 Sep 30. PMID: 32936614.

LC/MS-QTOF

The column used was a 1.8-µm C18 Rapid Resolution HT (2.1 × 100 mm). Chromatographic separation was performed at 40℃using 1.8-µm C18 Rapid Resolution HT (2.1 × 100 mm) with (A) 0.1 % formic acid in dH20 and (B) 0.1 % formic acid in acetonitrile for positive mode ionization. Meanwhile, for negative mode ionization, the mobile phase used was (A) 0.1 % ammonium formate in dH20 and (B) acetonitrile. The total running time for both ionization modes was 30 min. The LC condition was re-equilibrated for 2 min before starting a new injection. The sample injection volume was set at 2 µL, and the flow rate of the mobile phase was set at 0.25 mL/min. The mass spectrometer was operated in the positive ESI mode with an optimum gas temperature of 325 ℃, gas flow rate of 11 L/min, and nebulizer at 35 psi. The samples (100 mg) were dissolved in 10 mL of 90 % ethanol to produce a concentration of 10 mg/mL. The sample was vortexed for 60 s until completely dissolved. Then, the samples were further diluted to a concentration of 1 mg/mL. Thereafter, all samples were filtered using a 0.22-µm pore size syringe filter before analysis.

来源文献：Aishah Baharuddin S, Nadiah Abd Karim Shah N, Saiful Yazan L, Abd Rashed A, Kadota K, Al-Awaadh AM, Aniza Yusof Y. Optimization of Pluchea indica (L.) leaf extract using ultrasound-assisted extraction and its cytotoxicity on the HT-29 colorectal cancer cell line. Ultrason Sonochem. 2023 Nov 23;101:106702. doi: 10.1016/j.ultsonch.2023.106702. Epub ahead of print. PMID: 38041881.

Zhuang M, et al. Drug Des Devel Ther. 2018 Apr 23;12:955-966.