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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20
- 保质期:
询客服
- 英文名:
一步法实时荧光定量试剂盒
- 库存:
999
- 供应商:
北京索莱宝
- 规格:
50T/200T
| 规格: | 50T | 产品价格: | ¥580.0 |
|---|---|---|---|
| 规格: | 200T | 产品价格: | ¥2180.0 |
| 别名 | Taqman一步法荧光定量PCR试剂盒 一步法实时荧光定量试剂盒 |
|---|---|
| 英文名称 | TaqMan One Step RT-qPCR Kit |
| 储存条件 | -20℃ |
| 单位 | 支 |
本产品是采用探针法(TaqMan,Molecular Beacon等)进行一步法Real-Time RTqPCR的专用试剂盒。使用本产品进行RealTime RT-qPCR反应时,逆转录和定量PCR在同一反应体系中进行,反应过程中无需添加试剂,无需打开管盖,避免了污染的同时提高了实验效率。本产品的检测灵敏度高,荧光信号强,信噪比高,非常适合于RNA病毒等微量RNA的检测。其所包含的特殊缓冲系统能使逆转录酶与DNA聚合酶同时发挥最大功效,提高反应效率。使用本产品可以得到更宽广的线性范围,对目的基因定量更准确,重复性好,可信度高。
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文献和实验Quantitation of MicroRNAs by Real-Time RT-qPCR
similarity, a number of quantitative RT-qPCR-based miRNA quantitation methods have been introduced since 2004. The most commonly used methods are stem-loop reverse transcription (RT)-based TaqMan� MicroRNA assays and arrays. The high sensitivity
Stem-Loop RT-qPCR for MicroRNA Expression Profiling
Quantification of the microRNAs (miRNAs) in cells or tissues is a crucial step in understanding their biological functions. Development of the stem-loop reverse transcription procedure and TaqMan� miRNA assays enables accurate detection
TaqMan Primer and Probe Design
Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit Instruction Design of Probes Keep the G-C content in the 20 to 80% range. Avoid runs of an identical nucleotide. This is especially true for guanine, where runs










