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- 文献和实验
- 技术资料
- 保存条件:
避免反复冻融。2-8°C不超过一个月,-80°C不超过2475个月
- 规格:
50ug/100ug
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU/µg(LAL法测定)
- 亚细胞定位细胞核, 线粒体, 细胞质
- 预测分子量40.5kDa
- 实际分子量41kDa(差异分析请参阅说明书)
- 片段与标签Ala2~Asp339 with N-terminal His Tag
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性状冻干粉
- 纯度> 97%
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文献和实验Biochemical Studies on Human Rad51-Mediated Homologous Recombination
Rad51-mediated pairing between homologous DNA sequences during homologous recombination (HR) plays pivotal roles in DNA double-strand break repair. The multi-step process occurs through cooperation of Rad51 and a number of accessory protein
Assay for Human Rad51-Mediated DNA Displacement Loop Formation
同源重组(Homologus Recombination) 是指发生在姐妹染色单体(sister chromatin) 之间或同一染色体上含有同源序列的DNA分子之间或分子之内的重新组合。同源重组需要一系列的蛋白质催化,如原核生物细胞内的RecA、RecBCD、RecF、RecO、RecR等;以及真核生物细胞内的Rad51、Mre11-Rad50等等。 在人类细胞中,同源重组由Rad51介导,在有ATP的条件下,Rad51聚合在单链DNA上,形成一个核蛋白
In Vitro Assays for DNA Pairing and Recombination-Associated DNA Synthesis
/Rad51 family of proteins are the key enzymes in this homology-directed repair pathway, as they perform DNA strand invasion and exchange, in concert with a host of ancillary factors. In vitro, the RecA/Rad51 family of proteins share similar enzymatic
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