Rnase A

RNase inhibitors and RNases

  Question   1.What are the differences among RNase H, RNase A, RNase B and RNase C? 2.In your cDNA kits, RNase H is added in the second strand reaction to produce more nicked RNA as primers for DNA synthesis. In this repect

用RNase III制备siRNA库来诱发RNAi

% ），这使得化学合成siRNA 的成本升高。体外转录制备长片断RNA 并不难，但是由于研究表明长片断的双链RNA （>29bps ）在哺乳动物细胞中通常会引发抗病毒反应―― 一种非特异基因表达抑制，而目前体外转录往往无法制备小于29bp 的小分子siRNA s 。一个新的研究发现，用RNase III 降解长片断双链RNA 成为siRNA s 库也能够有效诱导特异的基因沉默，这样可以避免了筛选有效siRNA s 的漫长过程，从而快速，经济的实现特定基因表达的沉默。在线虫、果蝇和其他一些物种的RNA 干扰

How to Maintain an RNase-free Lab

  How to Maintain an RNase-free Lab Let's admit it, everyone who has worked with RNA has, at some point, been affected by RNase contamination. Sources of contaminating RNase include procedures that use RNase such as plasmid preps