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Recombinant Human CD19, His-tagged
20ug
Cat.No.: | CD19-150H |
Product Overview: | Recombinant Human CD19 Protein, With C-His Tag (rhCD19) Pro 20 - Lys 291 (Accession # AAH06338) was produced in human 293 cells (HEK293). |
Description: | B-lymphocyte antigen CD19 is also known as CD19 (Cluster of Differentiation 19), is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. As on T cells, several surface molecules form the antigen receptor and form a complex on B lymphocytes. The (almost) B cell-specific CD19 phosphoglycoprotein is one of these molecules. The others are CD21 and CD81. These surface immunoglobulin (sIg)-associated molecules facilitate signal transduction. On living B cells, anti-immunoglobulin antibody mimicking exogenous antigen causes CD19 to bind to sIg and internalize with it. The reverse process has not been demonstrated, suggesting that formation of this receptor complex is antigen-induced. This molecular association has been confirmed by chemical studies. Mutations in CD19 are associated with severe immunodeficiency syndromes characterized by diminished antibody production. CD19 has been shown to interact with: CD81, CD82, Complement receptor 2, and VAV2. |
Source: | HEK293 |
Species: | Human |
Tag: | His |
Predicted N Terminal: | Pro 20 |
Form: | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization. |
Bio-activity: | Measured by its binding ability in a functional ELISA. Immobilized rhCD19 at 2 μg/ml ( 100 μl/well ) can bind biotinylated human CD9 with a linear ranger of 0.025 - 0.5 μg/ml.Validated Blinatumomab* binding in a functional ELISA. |
Molecular Mass: | rhCD19 is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 31.6 kDa. The predicted N-terminus is Pro 20. DTT-reduced Protein migrates as 40-65 kDa in SDS-PAGE due to different extent glycosylation. |
Endotoxin: | Less than 1.0 EU per μg of the rhCD19 by the LAL method. |
Purity: | >95% as determined by SDS-PAGE. |
Applications: | CD Antigens |
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