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- ant-700
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
for future use below -18°C
- 保质期：
See instructions
- 英文名：
SSU72
- 库存：
常规产品有备货
- 供应商：
上海经科化学科技有限公司
- CAS号：
无
- 规格：
5ug/20ug/100ug
| 规格： | 5ug | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 20ug | 产品价格： | ￥2415.0 |
| 规格： | 100ug | 产品价格： | ￥7500.0 |
CATALOGUE NUMBER
ANT-700
SYNONYMS
SSU72 RNA polymerase II CTD phosphatase homolog (S. cerevisiae), HSPC182, CTD phosphatase SSU72, Ssu72 RNA polymerase II CTD phosphatase homolog (yeast), PNAS-120, RNA polymerase II subunit A C-terminal domain phosphatase SSU72, EC 3.1.3.16.
TYPE
INTRODUCTION
SSU72 is an extremely conserved homologue of yeast Ssu72, a CTD phosphatase and a component of the polyadenylation/termination machinery. SSU72 interacts with TFIIB, Rb and DNAM-1 and operates to catalyze the dephosphorylation of target proteins, and taking part in RNA processing and termination via dephosphorylation of Pol II. SSU72 is found in multiple alternatively spliced isoforms.
CLONE
PAT66D10AT.
PHYSICAL APPEARANCE
IMMUNOGEN
Anti-human SSU72 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human SSU72 amino acids 1-194 purified from E. coli.
IG SUBCLASS
Mouse IgG2b heavy chain and κ light chain.
PURIFICATION METHOD
SSU72 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
FORMULATION
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
STORAGE PROCEDURES
STABILITY / SHELF LIFE
APPLICATIONS
SSU72 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
SAFETY DATA SHEET
SDS
USAGE
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文献和实验SSU72 is an extremely conserved homologue of yeast Ssu72, a CTD phosphatase and a component of the polyadenylation/termination machinery. SSU72 interacts with TFIIB, Rb and DNAM-1 and operates to catalyze the dephosphorylation of target proteins, and taking part in RNA processing and termination via dephosphorylation of Pol II. SSU72 is found in multiple alternatively spliced isoforms.
96-well RNA In Situ Hybridization Protocol
. at RT. 19. Remove PBT, add 200μlPBT + 5% Normal Goat Serum + anti-digoxigenin Alkaline Phosphatase (1:2000 dilution), rock 2 hrs at RT. 20. Rinse twice with PBT . 21. Wash embryos 9 X for 10 min. in PBT. 22. Rinse twice with 200μlAlkaline Phosphatase
Intron-Mediated RNA Interference, Intronic MicroRNAs, and Applications
of intronic miRNAs have been identified in C. elegans , mouse, and human cells. However, neither function nor application has been reported. Here, we show that, for the first time, intron-derived miRNAs are able to induce RNA interference not only in human
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
技术资料