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- 文献和实验
- 技术资料
- 保存条件:
for future use below -18°C
- 保质期:
See instructions
- 英文名:
bThrombin
- 库存:
常规产品有备货
- 供应商:
上海经科化学科技有限公司
- CAS号:
无
- 规格:
2000U/10000U/100,000U
| 规格: | 2000U | 产品价格: | ¥1080.0 |
|---|---|---|---|
| 规格: | 10000U | 产品价格: | ¥2450.0 |
| 规格: | 100,000U | 产品价格: | ¥8100.0 |

CATALOGUE NUMBER
PRO-447
SYNONYMS
Coagulation Factor II (Thrombin), Prepro-Coagulation Factor II, EC 3.4.21.5, RPRGL2, THPH1, Coagulation Factor II, Prothrombin B-Chain, Serine Protease, Prothrombin, EC 3.4.21, PT, F2.
INTRODUCTION
SOURCE
PHYSICAL APPEARANCE
Sterile Filtered beige lyophilized (freeze-dried) powder.
FORMULATION
Lyophilized freeze dry powder formulated with glycine, CaCl2 and sodium chloride, PH 6.8.
SOLUBILITY
It is recommended to reconstitute the lyophilized Thrombin in 0.9% NaCl.
BIOLOGICAL ACTIVITY
164 US unites/mg protein.
STABILITY
Lyophilized Thrombin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Thrmbin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
SAFETY DATA SHEET
SDS
USAGE
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文献和实验Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.
Isolation of Microtubules (Bovine Brain)
LEVEL II Materials Freshly removed bovine brain 2 Wire sieve (tea strainer) Microtubule buffer (MT buffer) 0.1 M MES (2-(N-Morphilino)ethanesulfonic acid) 1 mM EGTA (Ethylene Glycol-bis
Generation of thrombin has been established as the critical process leading to coagulation in vivo. Indeed, ex vivo markers of thrombin generation in patients have been useful in detecting thrombosis, while many standard global clot-time
Thrombin clotting time (TCT) is a coagulation assay used to diagnose congenital and acquired fibrinogen deficiency (Adcock et al., Coagulation handbook, Esoterix Coagulation, Austin, TX, 2002), as well as to identify contamination by heparin
技术资料








