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- pro-226
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
for future use below -18°C
- 保质期：
See instructions
- 英文名：
SNRNP25
- 库存：
常规产品有备货
- 供应商：
上海经科化学科技有限公司
- CAS号：
无
- 规格：
5ug/20ug/1mg
| 规格： | 5ug | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 20ug | 产品价格： | ￥2415.0 |
| 规格： | 1mg | 产品价格： | ￥60858.0 |
CATALOGUE NUMBER
PRO-226
SYNONYMS
U11/U12 small nuclear ribonucleoprotein 25 kDa protein, U11/U12 snRNP 25 kDa protein, U11/U12-25K, Minus-99 protein, SNRNP25, C16orf33.
INTRODUCTION
SNRNP25 is a member of the small nuclear ribonucleoprotein core protein family. SNRNP25 is a component of the U12-type spliceosome which is found in some eukaryotes and removes U12-type introns that are rare and have distinct splice consensus signals. The U12-type spliceosome is comprised of several small nuclear RNAs and associated proteins.
DESCRIPTION
SNRNP25 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids (1-132 a.a.) and having a molecular mass of 17.4kDa.
SNRNP25 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
SOURCE
PHYSICAL APPEARANCE
FORMULATION
SNRNP25 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 2mM DTT and 0.2M NaCl.
STABILITY
SNRNP25 Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.
PURITY
AMINO ACID SEQUENCE
MG.S.SHHHHHH SSGLVPRGSH MDVFQEGLAM VVQDPLLCDL PIQVTLEEVN SQIALEYGQA MTVRVCKMDG EVMPVVVVQS ATVLDLKKAI QRYVQLKQER EGGIQHISWS YVWRTYHLTS AGEKLTEDRK KLRDYGIRNR DEVSFIKKLR QK.
SAFETY DATA SHEET
SDS
USAGE
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文献和实验SNRNP25 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids (1-132 a.a.) and having a molecular mass of 17.4kDa.
SNRNP25 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Purification of U Small Nuclear Ribonucleoprotein Particles
The spliceosome is the catalytic entity that removes the introns from the primary transcripts in eukaryotes. The spliceosome consists of four small nuclear ribonucleoproteins (snRNPs), named U1,U2, U4/U6, and U5 snRNP, and numerous non-snRNP
Preparation of Heterogeneous Nuclear Ribonucleoprotein Complexes
processing pathway alters the structure of the underlying RNA substrate. Therefore, hnRNP complexes within the cell, although probably unique for each transcript, are not uniform structures, such as a ribosomal subunit or a particular small nuclear
Antisense oligonucleotides have been used to study the structure and function of small nuclear ribonucleoprotein (snRNP) complexes and were adapted and modified for the purification of a variety of RNPs. We describe methods for recombinant
技术资料