- ￥840 - 27000
- Prospecbio
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- pro-1925
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
for future use below -18°C
- 保质期：
See instructions
- 英文名：
SPA His
- 库存：
常规产品有备货
- 供应商：
上海经科化学科技有限公司
- CAS号：
无
- 规格：
10mg/100mg/1gram
| 规格： | 10mg | 产品价格： | ￥840.0 |
|---|---|---|---|
| 规格： | 100mg | 产品价格： | ￥4500.0 |
| 规格： | 1gram | 产品价格： | ￥27000.0 |
CATALOGUE NUMBER
PRO-1925
SYNONYMS
INTRODUCTION
DESCRIPTION
SOURCE
PHYSICAL APPEARANCE
FORMULATION
SOLUBILITY
STABILITY
PURITY
(a) Analysis by HPLC.
(b) Analysis by SDS-PAGE.
AMINO ACID SEQUENCE
SAFETY DATA SHEET
SDS
USAGE
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文献和实验SPA Recombinant produced in E.Coli is a single non-glycosylated polypeptide chain fused with 6×His tag at C-terminus. SPA is comprised of 5 IgG-binding domains E-D-A-B-C aligned in series containing 306 amino acids and having a molecular mass of 34.7kDa containing little or no carbohydrate. Cell wall binding region, cell membrane binding region and albumin binding region were removed to ensure the highest specific IgG binding.
One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin
The 6xHis/Ni-NTA system is a fast and versatile tool for the affinity purification of recombinant proteins and antigenic peptides. It is based on the high-affinity binding of six consecutive histidine residues (the 6xHis tag) to immobilized
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
and thus fusion proteins. For example there might be sequence for a fluorescent protein such as GFP or a peptide tag such as HA upstream of MCS (and downstream of promoter) so that an insert in frame will lead to the generation of a fusion protein, an N-terminal
For recombinant expression of extracellular matrix (ECM) proteins or their individual domains, the use of transformed mammalian cells offers two major advantages. First, eukaryotic expression can be expected under optimum conditions
技术资料