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- pro-171
- 2026年01月20日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
for future use below -18°C
- 保质期：
See instructions
- 英文名：
MYL6
- 库存：
常规产品有备货
- 供应商：
上海经科化学科技有限公司
- CAS号：
无
- 规格：
2ug/10ug/1mg
| 规格： | 2ug | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 10ug | 产品价格： | ￥2415.0 |
| 规格： | 1mg | 产品价格： | ￥87906.0 |
CATALOGUE NUMBER
PRO-171
SYNONYMS
INTRODUCTION
DESCRIPTION
MYL6 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 171 amino acids (1-151 a.a.) and having a molecular mass of 19.1kDa. The MYL6 is purified by proprietary chromatographic techniques.
SOURCE
PHYSICAL APPEARANCE
FORMULATION
STABILITY
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
PURITY
AMINO ACID SEQUENCE
MG.S.SHHHHHH SSGLVPRGSH MCDFTEDQTA EFKEAFQLFD RTGDGKILYS QCGDVMRALG QNPTNAEVLK VLGNPKSDEM NVKVLDFEHF LPMLQTVAKN KDQGTYEDYV EGLRVFDKEG NGTVMGAEIR HVLVTLGEKM TEEEVEMLVA GHEDSNGCIN YEELVRMVLN G.
SAFETY DATA SHEET
SDS
USAGE
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文献和实验MYL6 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 171 amino acids (1-151 a.a.) and having a molecular mass of 19.1kDa. The MYL6 is purified by proprietary chromatographic techniques.
Myosin Light Chain Preparation
in lyophilizer bottles and freeze in liquid nitrogen.Put on lyophilizer and leave until dry (overnight or over weekend). Day 5 22.Resuspend separate light chain pools 1 and 2 in a small volume of Dialyzing Solution G.Pipette into dialyzing tubing (size #20
Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
构成肌球蛋白的低分子量（1.5— 2.5万）的亚单位。一分子肌球蛋白有 4个。肌球蛋白有在腺苷三磷酸酶（ ATP ase）作用中不可缺少的基本 L链和调节 ATP ase作用的调节 L链。从兔的白肌除去调节 L链（ L2 ：由 168个氨基酸所成）不影响肌球蛋白的作用。基本 L链（ L1 ∶ 190个氨基酸、 L3 ∶ 149个氨基酸）是必要的。兔白肌的肌球蛋白分子，根据 L链的不同而分为 3种，即每分子肌球蛋白中都含有两个 L2 ，其它两个则因种类不同而异，即分 L1
技术资料
