Recombinant Human Queuine TRNA

 Queuine TRNA-Ribosyltransferase Domain Containing 1, EC 2.4.2.29, Queuine TRNA-Ribosyltransferase Domain-Containing Protein 1, QTRTD1.

Queuine TRNA-Ribosyltransferase Domain Containing 1, also known as QTRTD1 act together with QTRT1 to form an active queuine tRNA-ribosyltransferase. QTRTD1 exchanges queuine for the guanine at the wobble position of tRNAs along with GUN anticodons (tRNA-Asp,-Asn,-His and -Tyr), in that way forming the hypermodified nucleoside queuosine.

QTRTD1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 438 amino acids (1-415 a.a) and having a molecular mass of 49.1kDa.
QTRTD1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile Filtered colorless solution.

QTRTD1 protein solution (0.25mg/ml) containing Phosphate buffered saline (pH7.4) 30% glycerol, 2mM DTT, 1mM EDTA and 0.1mM PMSF.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Greater than 95.0% as determined by SDS-PAGE.

MG./S-SHHHHHH SSGLVPRGSH MGSMKLSLTK VVNGCRLGKI KNLGKTGDHT MDIPGCLLYT KTGSAPHLTH HTLHNIHGVP AMAQLTLSSL AEHHEVLTEY KEGVGKFIGM PESLLYCSLH DPVSPCPAGY VTNKSVSVWS VAGRVEMTVS KFMAIQKALQ PDWFQCLSDG EVSCKEATSI KRVRKSVDRS LLFLDNCLRL QEESEVLQKS VIIGVIEGGD VMEERLRSAR ETAKRPVGGF LLDGFQGNPT TLEARLRLLS SVTAELPEDK PRLISGVSRP DEVLECIERG VDLFESFFPY QVTERGCALT FSFDYQPNPE ETLLQQNGTQ EEIKCMDQIK KIETTGCNQE ITSFEINLKE KKYQEDFNPL VRGCSCYCCK NHTRAYIHHL LVTNELLAGV LLMMHNFEHY FGFFHYIREA LKSDKLAQLK ELIHRQAS.

SDS

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