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Recombinant Lactate Dehydrogen

ase B
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  • ¥1080 - 15800
  • Prospecbio
  • 以色列
  • enz-279
  • 2026年01月05日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      for future use below -18°C

    • 保质期

      See instructions

    • 英文名

      LDHB

    • 库存

      常规产品有备货

    • 供应商

      上海经科化学科技有限公司

    • CAS号

    • 规格

      1mg/3mg/30mg

    规格:1mg产品价格:¥1080.0
    规格:3mg产品价格:¥2415.0
    规格:30mg产品价格:¥15800.0

    产品细节图片1

    CATALOGUE NUMBER

    ENZ-279

    SYNONYMS

    Lactate Dehydrogenase, LDH.

    INTRODUCTION

    Lactate dehydrogenase (LDH) is an enzyme(EC1.1.1.27) present in a wide variety of organisms, including plants and animals.
    A tetrameric enzyme that catalyses the interconversion of pyruvateand lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.

    DESCRIPTION

    The DNA encoding chicken LDH-B is cloned from cDNA library of chicken heart.

    SOURCE

    Escherichia Coli.

    PHYSICAL APPEARANCE

    Sterile lyophilized powder.

    FORMULATION

    The protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate.

    SOLUBILITY

    It is recommended to reconstitute the lyophilized LDH in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

    STABILITY

    Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C.
    Please prevent freeze-thaw cycles.

    PURITY

    Greater than 95.0% as determined by:
    (a) Analysis by RP-HPLC.
    (b) Analysis by SDS-PAGE.

    BIOLOGICAL ACTIVITY

    The specific activity was found to be 258 U/mg protein.

    UNIT DEFINITION

    One unit is defined as 1umol of NAD+ production per minute under the assay conditions (25°C, pH 7.0). Both transaminase activities include a-hydroxyglutarate dehydrogenase activity.

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    图标文献和实验
    该产品被引用文献

    Title: UDP-N-Acetylglucosamine 2-Epimerase/N-Acetylmannosamine Kinase (GNE) Binds to Alpha-Actinin 1: Novel Pathways in Skeletal Muscle?
    Publications:   PLoS One 3.6 (2008): e2477.

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    文献支持
    Recombinant Lactate Dehydrogenase B
    ¥1080 - 15800