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Recombinant Yeast Isocitrate D

ehydrogenase-1
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  • ￥1600 - 23000
  • Prospecbio
  • 以色列
  • enz-289
  • 2026年01月03日
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    上海经科化学科技有限公司
    16 年钻石会员

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    • 文献和实验
    • 技术资料
    • 保存条件：

      for future use below -18°C

    • 保质期：

      See instructions

    • 英文名：

      IDH1

    • 库存：

      常规产品有备货

    • 供应商：

      上海经科化学科技有限公司

    • CAS号：

      无

    • 规格：

      1mg/5mg/50mg

    规格：1mg产品价格：￥1600.0
    规格：5mg产品价格：￥4100.0
    规格：50mg产品价格：￥23000.0

     

    CATALOGUE NUMBER

    ENZ-289

    SYNONYMS

    Isocitrate dehydrogenase [NADP] cytoplasmic, EC 1.1.1.42, Cytosolic NADP-isocitrate dehydrogenase, Oxalosuccinate decarboxylase, IDH, NADP(+)-specific ICDH, IDP, PICD.

    INTRODUCTION

    Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle.

    DESCRIPTION

    Recombinant Saccharomyces Cerevisiae ICDH (NADP) derived from yeast host cells by using over-expression system, is full length same as designated ICD1 from Saccharomyces Cerevisiae. The N-terminal amino acid Phenylalanine residue next to Met is substituted with Alanine for overexpression.
    The ICDH is purified by proprietary chromatographic techniques.

    SOURCE

    Yeast cells.

    PHYSICAL APPEARANCE

    Sterile Filtered clear solution.

    FORMULATION

    One ml of solution contains 0.075 mol/l KPO4, 50% Glycerol, pH 7.1.

    STABILITY

    Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Avoid multiple freeze-thaw cycles.

    PURITY

    Greater than 90% as determined by SDS-PAGE.

    BIOLOGICAL ACTIVITY

    The specific activity was found to be 115 U/mg.

    UNIT DEFINITION

    One unit is defined as 1 µmol of NAD+ production per minute under the assay conditions (25°C, pH 7.5).

    SAFETY DATA SHEET

    SDS

    USAGE

    Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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    图标文献和实验
    该产品被引用文献

    Recombinant Saccharomyces Cerevisiae ICDH (NADP) derived from yeast host cells by using over-expression system, is full length same as designated ICD1 from Saccharomyces Cerevisiae. The N-terminal amino acid Phenylalanine residue next to Met is substituted with Alanine for overexpression.
    The ICDH is purified by proprietary chromatographic techniques.

    相关实验

    • 异柠檬酸脱氢酶 isocitrate dehydrogenase

      　　异柠檬酸脱氢酶 isocitrate dehydrogenase 有两种类型的酶：要求以 NAD为辅酶的酶（ EC1． 1． 1． 41）和要 NADP的酶（ EC1． 1． 1． 42），两者催化同一反应。 　　异柠檬酸 NAD（ P） α -酮戊二酸 CO2 NAP（ P） H H4 　　在三羧酸循环中正常起作用的是要求 NAD为辅酶的酶，此酶是为 ADP激活的变构酶。  

    • Expression of Recombinant Matrix Metalloproteinases in Yeast

      Deciding what system to use for recombinant matrix metalloproteinase (MMP) expression is often a matter of practicality (i.e., simplicity, cost effectiveness, time considerations). Of course, the final judgment of practicality is determined

    • Setting Up a Bioreactor for Recombinant Protein Production in Yeast

      Scale-up from shake flasks to bioreactors allows for the more reproducible, high-yielding production of recombinant proteins in yeast. The ability to control growth conditions through real-time monitoring facilitates further optimization

    查看更多相关实验 >

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    文献支持
    Recombinant Yeast Isocitrate Dehydrogenase-1
    ￥1600 - 23000