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Bovine Alkaline Phosphatase

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  • ¥1600 - 33810
  • Prospecbio
  • 以色列
  • enz-322
  • 2025年12月31日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      for future use below -18°C

    • 保质期

      See instructions

    • 英文名

      bAlkaline Phosphatase

    • 库存

      常规产品有备货

    • 供应商

      上海经科化学科技有限公司

    • CAS号

    • 规格

      1mg/5mg/30mg

    规格:1mg产品价格:¥1600.0
    规格:5mg产品价格:¥7245.0
    规格:30mg产品价格:¥33810.0

     

    CATALOGUE NUMBER

    ENZ-322

    SYNONYMS

    EC 3.1.3.1, IAP, AP, ALPI, Intestinal-type alkaline phosphatase, Intestinal alkaline phosphatase.

    DESCRIPTION

    The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.

    Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.

    SOURCE

    Calf Intestine.

    PHYSICAL APPEARANCE

    Sterile Filtered clear colorless solution.

    FORMULATION

    50% glycerol, 5mM MgCl2, 0.1mM ZnCl2 and 5mM TRIS, pH 7.0.

    STABILITY

    AP should be stored at 4°C.
    Please Do-Not freeze.

    PURITY

    95% pure by Gel Filtration.

    BIOLOGICAL ACTIVITY

    >1500 U/mg (pH 9.6), 25°C and 0.025M glycin, 10% glycerol as buffer.

    SAFETY DATA SHEET

    SDS

    USAGE

    Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
     

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    图标文献和实验
    该产品被引用文献

    The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.

    Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.

    相关实验
    • ALKALINE PHOSPHATASE

      in solution c. for 5-15 minutes at room temperature. Wash, counterstain in haematoxylin, wash. Mount in phosphate buffered glycerine jelly RESULTS   Alkaline phosphatase activity - Red Nuclei - Blue  

    • Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity

        Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity 1. Obtain embryo samples, tube of AP substrate buffer and tube of phosphate buffered saline ( PBS ) for each group. Allow embryos

    • Alkaline phosphatase staining

      1.1 General information Endothelial cells possess an endogenous alkaline phosphatase (AP) activity. The enzymatic activity of AP is not restricted to the endothelial lineage. In medaka embryos, somite cells during early developmental stages

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    文献支持
    Bovine Alkaline Phosphatase
    ¥1600 - 33810