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- 文献和实验
- 技术资料
- 保存条件:
for future use below -18°C
- 保质期:
See instructions
- 英文名:
bAlkaline Phosphatase
- 库存:
常规产品有备货
- 供应商:
上海经科化学科技有限公司
- CAS号:
无
- 规格:
1mg/5mg/30mg
| 规格: | 1mg | 产品价格: | ¥1600.0 |
|---|---|---|---|
| 规格: | 5mg | 产品价格: | ¥7245.0 |
| 规格: | 30mg | 产品价格: | ¥33810.0 |
CATALOGUE NUMBER
ENZ-322
SYNONYMS
DESCRIPTION
The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.
Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.
SOURCE
PHYSICAL APPEARANCE
FORMULATION
STABILITY
Please Do-Not freeze.
PURITY
BIOLOGICAL ACTIVITY
>1500 U/mg (pH 9.6), 25°C and 0.025M glycin, 10% glycerol as buffer.
SAFETY DATA SHEET
SDS
USAGE
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文献和实验The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.
Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.
in solution c. for 5-15 minutes at room temperature. Wash, counterstain in haematoxylin, wash. Mount in phosphate buffered glycerine jelly RESULTS Alkaline phosphatase activity - Red Nuclei - Blue
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity 1. Obtain embryo samples, tube of AP substrate buffer and tube of phosphate buffered saline ( PBS ) for each group. Allow embryos
1.1 General information Endothelial cells possess an endogenous alkaline phosphatase (AP) activity. The enzymatic activity of AP is not restricted to the endothelial lineage. In medaka embryos, somite cells during early developmental stages
技术资料









