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Recombinant Human Thrombopoiet

in, HEK
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  • ¥1080 - 96600
  • Prospecbio
  • 以色列
  • cyt-115
  • 2025年12月27日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      for future use below -18°C

    • 保质期

      See instructions

    • 英文名

      TPO, HEK

    • 库存

      常规产品有备货

    • 供应商

      上海经科化学科技有限公司

    • CAS号

    • 规格

      2ug/10ug/1mg

    规格:2ug产品价格:¥1080.0
    规格:10ug产品价格:¥2415.0
    规格:1mg产品价格:¥96600.0

     

    CATALOGUE NUMBER

    CYT-115

    SYNONYMS

    Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, C-mpl ligand, ML, Megakaryocyte growth and development factor, MGDF, TPO, MKCSF, MPLLG, MGC163194, THPO.

    INTRODUCTION

    Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. It stimulates the production and differentiation of megakaryocytes, the bone marrow cells that fragment into large numbers of platelets. 

    DESCRIPTION

    Thrombopoietin Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight range of 80-85kDa due to glycosylation.
    The TPO is purified by proprietary chromatographic techniques.

    SOURCE

    HEK.

    PHYSICAL APPEARANCE

    Sterile Filtered White lyophilized (freeze-dried) powder.

    FORMULATION

    The TPO protein was filtered (0.2µm) and lyophilized from 0.74mg/ml in 1xPBS.

    SOLUBILITY

    It is recommended to reconstitute the lyophilized Thrombopoietin in sterile PBS containing 0.1% endotoxin-free recombinant HSA.

    STABILITY

    Lyophilized Thrombopoietin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TPO should be stored at 4°C between 2-7 days and for future use below -18°C.
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please prevent freeze-thaw cycles.

    PURITY

    Greater than 95% as obsereved by SDS-PAGE.

    BIOLOGICAL ACTIVITY

    The activity was determined by the dose-dependent stimulation of the proliferation of MO7e cells, the ED50 is 1.15ng/ml.

    AMINO ACID SEQUENCE

    SPAPPACDLRVLSKLLRDSHVLHSRLSQCPEVHPLPTPVLLPAVDFSLG EWKTQMEETKAQDILGAVTLLLEGVMAARGQLGPTCLSSLLGQLSGQV RLLLGALQSLLGTQLPPQGRTTAHKDPNAIFLSFQHLLRGKVRFLMLVGG STLCVRRAPPTTAVPSRTSLVLTLNELPNRTSGLLETNFTASARTTGSGLLK WQQGFRAKIPGLLNQTSRSLDQIPGYLNRIHELLNGTRGLFPGPSRRTLGAP DISSGTSDTGSLPPNLQPGYSPSPTHPPTGQYTLFPLPPTLPTPVVQLHPLLPDPSAPTPT

    PTSPLLNTSYTHSQNLSQEG

    SAFETY DATA SHEET

    SDS

    USAGE

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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    图标文献和实验
    该产品被引用文献

    Thrombopoietin Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight range of 80-85kDa due to glycosylation.
    The TPO is purified by proprietary chromatographic techniques.

    相关实验
    • Thrombopoietin Bioassay

      in emergencies such as bleeding. Forty years ago, it was recognized that a humoral regulator circulating in thrombocytopenic animals was able to stimulate platelet production in normal recipients (1 ). Termed thrombopoietin (TPO), the properties of this activity

    • Construction of Recombinant Human Cytomegalovirus

      The use of reverse genetics to generate recombinant viruses allows the researcher to investigate the exact functional significance of particular viral genes during the virus life cycle, by means of their deletion or modification in the viral

    • Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells

      Due to its excellent transfectability, the human embryonic kidney (HEK) 293 cell line is widely used as an in vitro model system for transfection experiments. Particle bombardment, or biolistics technology, provides a physical transfection

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    Recombinant Human Thrombopoietin, HEK
    ¥1080 - 96600