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Recombinant Human Stem Cell Fa

ctor, Sf9
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  • ￥1080 - 80000
  • Prospecbio
  • 以色列
  • cyt-421
  • 2025年12月27日
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    上海经科化学科技有限公司
    15 年钻石会员

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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件：

      for future use below -18°C

    • 保质期：

      See instructions

    • 英文名：

      SCF, Sf9

    • 库存：

      部分小规格有备货

    • 供应商：

      上海经科化学科技有限公司

    • CAS号：

      无

    • 规格：

      2ug/10ug/1mg

    规格：2ug产品价格：￥1080.0
    规格：10ug产品价格：￥2415.0
    规格：1mg产品价格：￥80000.0

    产品细节图片1

    CATALOGUE NUMBER

    CYT-421

    SYNONYMS

    Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.

    INTRODUCTION

    Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases.
    SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).

    DESCRIPTION

    Stem Cell Factor Human Recombinant produced in insect cells is a single, glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton.
    The SCF is fused to a C-terminal His-tag (6xHis) and purified by proprietary chromatographic techniques.

    SOURCE

    Sf9, Insect Cells.

    PHYSICAL APPEARANCE

    Sterile Filtered White lyophilized (freeze-dried) powder.

    FORMULATION

    The protein is supplied in 1xPBS, pH 7.4.

    SOLUBILITY

    It is recommended to reconstitute the lyophilized Stem Cell Factor in 10mM acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    STABILITY

    Lyophilized KIT ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please prevent freeze-thaw cycles.

    PURITY

    Greater than 98.0% as determined by analysis by SDS-PAGE.

    BIOLOGICAL ACTIVITY

    The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is typically 1-5 ng/ml.

    PROTEIN CONTENT

    Protein quantitation was carried out by two independent methods:
    1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
    2. Analysis by RP-HPLC, using a standard solution of Stem Cell Factor as a Reference Standard.

    SAFETY DATA SHEET

    SDS

    USAGE

    Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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    图标文献和实验
    该产品被引用文献

    Stem Cell Factor Human Recombinant produced in insect cells is a single, glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton.
    The SCF is fused to a C-terminal His-tag (6xHis) and purified by proprietary chromatographic techniques.

    相关实验

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    • Secretion of Recombinant Human Insulin-Like Growth Factor I (IGF-I)

      The development of efficient recombinant protein production processes can be a critical factor in whether or not a pharmaceutical therapeutic protein can enter human clinical trials and ultimately the marketplace. This is especially true

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    文献支持
    Recombinant Human Stem Cell Factor, Sf9
    ￥1080 - 80000