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Recombinant Human Interleukin-

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  • ￥1080 - 45885
  • Prospecbio
  • 以色列
  • cyt-210
  • 2025年12月25日
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    上海经科化学科技有限公司
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    • 技术资料
    • 保存条件：

      for future use below -18°C

    • 保质期：

      See instructions

    • 英文名：

      IL3

    • 库存：

      部分小规格有备货

    • 供应商：

      上海经科化学科技有限公司

    • CAS号：

      无

    • 规格：

      2ug/10ug/1mg

    规格：2ug产品价格：￥1080.0
    规格：10ug产品价格：￥2415.0
    规格：1mg产品价格：￥45885.0

    产品细节图片1

    CATALOGUE NUMBER

    CYT-210

    SYNONYMS

    MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.

    INTRODUCTION

    IL3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

    DESCRIPTION

    Interleukin-3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. The IL-3 is purified by proprietary chromatographic techniques.

    SOURCE

    Escherichia Coli.

    PHYSICAL APPEARANCE

    Sterile Filtered White lyophilized (freeze-dried) powder.

    FORMULATION

    Lyophilized from a concentrated (1mg/ml) solution in water containing 0.3 mg/ml of NaHCO3.

    SOLUBILITY

    It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    STABILITY

    Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C.
    Please prevent freeze-thaw cycles.

    PURITY

    Greater than 98.0% as determined by:
    (a) Analysis by SEC-HPLC.
    (b) Analysis by SDS-PAGE.

    AMINO ACID SEQUENCE

    The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Thr-Gln.

    BIOLOGICAL ACTIVITY

    The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 10,000,000 IU/mg.

    PROTEIN CONTENT

    Protein quantitation was carried out by two independent methods:
    1. UV spectroscopy at 280 nm using the absorbency value of 0.84 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
    2. Analysis by RP-HPLC, using a standard solution of IL-3 as a Reference Standard.

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    图标文献和实验
    该产品被引用文献

    1.Title:Interleukin-10 overexpression in macrophages suppresses atherosclerosis in hyperlipidemic mice.
    Publication:Published online before print March 30, 2010, doi: 10.1096/fj.09-148155 August 2010 The FASEB Journal vol. 24 no. 8 2869-2880 .

    2.Title:A Method for the Selection of Human Embryonic Stem Cell Sublines with High Replating Efficiency After Single-Cell Dissociation.
    Publication:Article first published online: 24 AUG 2006 DOI:10.1634/stemcells.2005-0657 Copyright © 2006 AlphaMed Press

     
    3.Title: Selective Activation of Human Dendritic Cells by OM-85 through a NF-kB and MAPK Dependent Pathway
    Publications:  PloS one 8.12 (2013): e82867
     
    4. Title: Novel markers of normal and neoplastic human plasmacytoid dendritic cells
    Publication:  Blood 111.7 (2008): 3778-3792.
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