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Recombinant Human Adiponectin

glycosilated, HEK
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  • ¥1080 - 94185
  • Prospecbio
  • 以色列
  • cyt-434
  • 2025年07月18日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      for future use below -18°C

    • 保质期

      See instructions

    • 英文名

      Acrp30, HEK

    • 库存

      部分小规格有备货

    • 供应商

      上海经科化学科技有限公司

    • 规格

      2ug/10ug/1mg

    规格:2ug产品价格:¥1080.0
    规格:10ug产品价格:¥2415.0
    规格:1mg产品价格:¥94185.0

    产品细节图片1

    CATALOGUE NUMBER

    CYT-434

    SYNONYMS

    Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

    INTRODUCTION

    Adiponectin is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells (Adipokine). The protein belongs to the soluble defense collagen super family; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum.

    DESCRIPTION

    The Acrp30 Human Recombinant produced in HEK293 cells is fused with FLAG tag.

    SOURCE

    HEK293 (Human Embryonic Kidney cell line).

    BIOLOGICAL ACTIVITY

    In vitro gluconeogenesis assay in primary hepatocytes was performed, showing the Adiponectin human derived from mammalian cells can inhibit glucose production. The ED50 was ~6 µg/ml.

    PHYSICAL APPEARANCE

    White lyophilized (freeze-dried) powder.

    FORMULATION

    Filtered (0.4µm) and lyophilized from 0.5mg/ml in 50mM phosphate Buffer, 75mM NaCl, pH 7.4.

    SOLUBILITY

    Add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product not sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.

    STABILITY

    For long term, store lyophilized AdipoQ at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable for 24 months when stored at -20°C.

    PURITY

    Greater than 98% as determined by HPLC and SDS PAGE.

    AMINO ACID SEQUENCE

    ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHIVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTNDYKDDDDK.

    SAFETY DATA SHEET

    SDS

    USAGE

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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    图标文献和实验
    该产品被引用文献

    Adiponectin is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells (Adipokine). The protein belongs to the soluble defense collagen super family; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum.

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    文献支持
    Recombinant Human Adiponectin glycosilated, HEK
    ¥1080 - 94185