Mouse MMP2 ELISA Kit (Sandwich

ELISA) - LS-F24871

价格$690

品牌LSBio
检测范围156 - 10000 pg/ml
货号LS-F24871
产地美国
更新日期2025年07月11日

上海康朗生物科技有限公司

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Mouse MMP2 ELISA Kit (Sandwich ELISA) - LS-F24871

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Product Description

LS-F24871 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse MMP2 in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of MMP2 as low as 10 picograms per milliliter.

Specifications

Type

Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target

MMP2
Reactivity

Mouse
Specificity

For the detection and quantification of endogenous levels of natural and/or recombinant Mouse MMP2 proteins.
Manual

Intended Sample Types

Cell Culture Supernatants, Plasma, Serum
Format

96-Well Strip Plate
Detection

Colorimetric - 450nm (TMB)
Measurement

Quantitative
Detection Range

156 - 10000 pg/ml
Sensitivity

10 pg/ml
Storage

Short term: 4°C; Long term: see manual.
Quality Assurance

Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components

User Manual
96-well Microplate
Detection Reagents
Wash Buffer
Substrate
Stop Solution
Adhesive Plate Sealers

Background

Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown. Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability. The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.
Restrictions

For research use only.
Guarantee

This elisa kit carries the LSBio 100% Guarantee.
About MMP2

Uniprot: P08253 NCBI: NM_004530 NP_004521.1