Synonyms: |
EFE-184 |
Background: |
established from the ascitic fluid of a 69-year-old Caucasian woman with endometrial carcinoma in relapse in 1985 |
Species: |
human (Homo sapiens) |
Tissue: |
endometrium |
Disease: |
endometrium carcinoma |
Gender: |
N/A |
Morphology: |
epitheloid cells growing in monolayers |
Growth Mode: |
N/A |
Doubling Time: |
about 92 hours |
DNA Profile: |
N/A Cell Line Authentication Service |
Culture Medium: |
RPMI1640+10%FBS We strongly suggest to purchase the complete medium from |
Cryopreservation medium: |
90%FBS+10%DMSO |
Mycoplasma: |
negative in DAPI, microbiological culture, RNA hybridization assays |
Immunology: |
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin + |
Fingerprint: |
multiplex PCR of minisatellite markers revealed a unique DNA profile |
Species: |
confirmed as human with IEF of AST, NP |
Cytogenetics: |
human flat-moded hypertriploid karyotype with 1-2 supernumerary dm in most cells and 23% polyploidy - 80(72-81)<3n>XX, -X, +1, +2, -4, -5, +6, +8, +10, -13, +17, +19, +20, +21, +6mar - der(1)t(1;?)(p11;?)x2, del(2)(q24)x2, der(2)t(2;3)(q11;p11), del(12)(p13), der(16)t(16;?)(q21/23;?), der(17)t(7;17)(q11;p11), add(22)(p11)x2 |
Virus |
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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