- ￥1700 - 4000
- GeneTex
- 美国
- GTX108613
- 2025年07月10日
- WB, ICC/IF, IHC-Fr, IP
- Rabbit
- Polyodon spathula, Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the N-terminus region of human ZO-1. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Polyodon spathula, Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX108613
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-Fr, IP
- 浓度：
0.22 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
ZO-1
- 抗体英文名：
ZO-1 antibody [N1N2], N-term
- 抗体名：
ZO-1 抗体 [N1N2], N-term
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: ZO-1 stained by ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500.
ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min.
Green: ZO-1 stained by ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:200.
Blue: Hoechst 33342 staining.
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文献和实验Meng-Hui Zhang et al., Bioengineered 2022 (PMID:35412944)
Yu-Ting Yen et al., Int J Mol Sci 2021 (PMID:35008751)
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Xu G et al., Front Mol Neurosci 2019 (PMID:30814927)
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Xiao Ke et al., Int J Clin Exp Med 2019;12(6)
Zhang N et al., Cell Biosci 2019 (PMID:31114672)
Arevalo-Romero H et al., Gastroenterol Res Pract 2016 (PMID:27525003)
Li H et al., Toxicol Appl Pharmacol 2017 (PMID:29294303)
Ding X et al., Lab Invest 2018 (PMID:29330471)
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Xun Liu et al., Cancer Sci 2022 (PMID:35271750)
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to protect the antibody. These should be stored in smaller aliquots (about 1 ml) and frozen for long term storage though the protected solution is also stable for months at four degrees. Ascites fluid: This is a bit more problematic as it may contain
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