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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ABCA4. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX108371
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
IHC-P
- 浓度:
0.55 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
ABCA4
- 抗体英文名:
ABCA4 antibody [C3-2], C-term
- 抗体名:
ABCA4 抗体 [C3-2], C-term
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
ABCA4 antibody [C3-2], C-term detects ABCA4 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Red: ABCA4 stained by ABCA4 antibody [C3-2], C-term (GTX108371) diluted at 1:250.
Green: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验that likely serve as substrates for this transporter. In this procedure, monoclonal antibodies directed either against an epitope within ABCA4 (Rim 3F4 antibody) or against the 9 amino acid 1D4 epitope tag engineered onto the C-terminus
于研究。 性能参数 LIST OF ANTBODY PRODUCTS AP8401a BDP1 N-term Antibody AP8408b MEG2 C-term Antibody AP8451b PPP3CA C-term Antibody AP8449b CTDSP1 C-term Antibody AP8408a MEG2 N-term Antibody AP8451a PPP3CA N-term Antibody AP8449
和其他膜蛋白进行了优化。用Rho1D4系统纯化后的膜蛋白通过凝胶过滤以除去用于洗脱的多肽。使用此纯化系统的科研人员均反馈蛋白回收量均达到毫克级。(见表1)3,4,5,6,7,8纯化的膜蛋白可用于功能性研究纯化的蛋白可用于功能性研究,如配体结合的特性,蛋白与蛋白间的相互作用。例如,将标记过的ABCA4固定在载有Rho1D4抗体的基质上来确定它与天然配体(一种视网膜的加成物)的亲和结合特性,加入ATP后即释放2。又例如,CD81蛋白被整体固定在涂有Rho1D4抗体的平板上,它表现出和分离可溶蛋白片段与丙型
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