- ￥1700 - 4000
- GeneTex
- 美国
- GTX108356
- 2025年07月15日
- WB, ICC/IF, IP
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HES1. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX108356
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IP
- 浓度：
1.32 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
HES1
- 抗体英文名：
HES1 antibody [N1], N-term
- 抗体名：
HES1 抗体 [N1], N-term
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [N1], N-term (GTX108356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
MCF-7 whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [N1], N-term (GTX108356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HES1 antibody [N1], N-term detects HES1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: HES1 protein stained by HES1 antibody [N1], N-term (GTX108356) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
HES1 antibody immunoprecipitates HES1 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : Control with 2.5 μg of pre-immune rabbit IgG B : Immunoprecipitation of HES1 by 2.5 μg of HES1 antibody (GTX108356) 12% SDS-PAGE The immunoprecipitated HES1 protein was detected by HES1 antibody (GTX108356) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
HES1 antibody [N1], N-term detects HES1 protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
12% SDS-PAGE
HES1 antibody [N1], N-term (GTX108356) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Liu Z et al., Mol Med Rep 2021 (PMID:33355365)
Li X et al., Am J Chin Med 2021 (PMID:33371813)
Hsieh MC et al., Int J Mol Sci 2019 (PMID:31454988)
Chen J et al., Stem Cells Dev 2017 (PMID:29256825)
Obora K et al., Sci Rep 2017 (PMID:28240738)
Chiou HY et al., J Biomed Sci 2014 (PMID:24894488)
Li L et al., PLoS One 2014 (PMID:24760075)
In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important
in pEGFP-N1 is between the immediate early promoter of CMV (PCMV IE ) and the EGFP coding sequences. Genes cloned into the MCS will be expressed as fusions to the N-terminus of EGFP if they are in the same reading frame as EGFP and there are no intervening
to protect the antibody. These should be stored in smaller aliquots (about 1 ml) and frozen for long term storage though the protected solution is also stable for months at four degrees. Ascites fluid: This is a bit more problematic as it may contain
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