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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Thioredoxin. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX108263
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IP
- 浓度:
0.69 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Thioredoxin
- 抗体英文名:
Thioredoxin antibody [N3C3]
- 抗体名:
Thioredoxin 抗体 [N3C3]
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Sample(30 μg of whole cell lysate)
A:H1299
B:HeLa S3(GTX14654)
12% SDS PAGE
GTX108263 diluted at 1:3000
Trx antibody [N3C3] detects Trx protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Trx protein stained by Trx antibody [N3C3] (GTX108263) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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文献和实验Lin WC et al., RSC Adv. 2017 7()
Ueno H et al., Biol Pharm Bull 2014 (PMID:25087957)
Nagano N et al., Biomedicines 2020 (PMID:32244938)
In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important
Thioredoxin and Related Proteins as Multifunctional Fusion Tags for Soluble Expression in E. coli
(1 ). Recombinant proteins produced in E. coli sometimes retain the N-terminal initiator methionine residue, as they may be a poor substrate for the host methionine aminopeptidase (2 ). In addition, individual purification schemes must be devised for each native
Materials 0.1M NaHC03 pH9 DMSO NHS-Biotin (N-Hydroxysuccinimidobiotin, Sigma #H-1759) PBS Procedure Dialyze the sample against carbonate buffer. After dialysis, adjust
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