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ERR alpha antibody [N1], N-ter

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  • ¥1700 - 4000
  • GeneTex已认证
  • 美国
  • 2025年07月15日
  • WB, ICC/IF, IHC-P, IP, ChIP assay
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human ERR alpha. The exact sequence is proprietary.

    • 亚型

      IgG

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Polyclonal

    • 标记物

      Unconjugated

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      Human

    • 目录编号

      GTX108166

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Rabbit

    • 应用范围

      WB, ICC/IF, IHC-P, IP, ChIP assay

    • 浓度

      1.32 mg/ml (Please refer to the vial label for the specific concentration.)

    • 靶点

      ERR alpha

    • 抗体英文名

      ERR alpha antibody [N1], N-term

    • 抗体名

      ERR alpha 抗体 [N1], N-term

    • 规格

      100 μl/25 μl

    规格:100 μl产品价格:¥4000.0
    规格:25 μl产品价格:¥1700.0
    产品细节图片1

    ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ERR alpha protein by 2 μg of ERR alpha antibody (GTX108166) 7.5% SDS-PAGE The immunoprecipitated ERR alpha protein was detected by ERR alpha antibody (GTX108166) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

    产品细节图片2

    ERR alpha antibody [N1], N-term detects ERR alpha protein at nucleus by immunohistochemical analysis.
    Sample: Paraffin-embedded rat colon.
    ERR alpha stained by ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500.
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

    产品细节图片3

    Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

    产品细节图片4

    ERR alpha antibody [N1], N-term detects ERR alpha protein at nucleus by immunofluorescent analysis.
    Sample: MCF-7 cells were fixed in 4% PFA at RT for 15 min.
    Green: ERR alpha stained by ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500.
    Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

    产品细节图片5

    Sample(30 μg of whole cell lysate)
    A:293T
    B:A431(GTX27909)
    C:H1299
    12% SDS PAGE
    GTX108166 diluted at 1:500
    The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

    产品细节图片6

    Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

    产品细节图片7

    Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.

    产品细节图片8

    ERR alpha antibody [N1], N-term detects ERR alpha protein at cytoplasm and nucleus by immunohistochemical analysis.
    Sample: Paraffin-embedded mouse intestine.
    ERR alpha stained by ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500.
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

    产品细节图片9

    Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

    产品细节图片10

    ERR alpha antibody [N1], N-term detects ERR alpha protein at cytoplasm and nucleus by immunohistochemical analysis.
    Sample: Paraffin-embedded mouse stomach.
    ERR alpha stained by ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500.
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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    图标文献和实验
    该产品被引用文献
    Application Reference
    Cai Q et al., Oncogene 2013 (PMID:22665055)
    Nowi?ska K et al., Int J Mol Sci 2022 (PMID:36430689)
    Panpan Dong et al., Exp Ther Med 2022 (PMID:35069845)
    Cerutti C et al., Sci Rep 2022 (PMID:35264626)
    Tribollet V et al., PLoS One 2016 (PMID:27227989)
    Tsushida K et al., Biochem Biophys Res Commun 2018 (PMID:29545177)
    Carnesecchi J et al., PLoS One 2017 (PMID:29190800)
    Puigserver P et al., Mol Cancer Res 2017 (PMID:28596418)
    Carnesecchi J et al., Proc Natl Acad Sci U S A 2017 (PMID:28348226)
    Sailland J et al., Proc Natl Acad Sci U S A 2014 (PMID:25288732)
    Carnesecchi J et al., PLoS One 2015 (PMID:25781607)
    Kammerer M et al., Mol Cell Endocrinol 2013 (PMID:23403054)
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    ¥1700 - 4000