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文献和实验Chemiluminescent Detection of Senescence-Associated Galactosidase
Identifying molecules that serve as markers for cell aging is a goal that has been pursued by several groups. Senescence-associated β galactosidase (SA-βgal) staining is broadly used and very easily detected. β-gal is a lysosomal enzyme
Methods to Detect Biomarkers of Cellular Senescence: The Senescence-Associated -Galactosidase Assay
phenotype, a senescence associated β-galactosidase (SA-β-gal), which is detected by histochemical staining of cells using the artificial substrate X-gal. The presence of the SA-β-gal biomarker is independent of DNA synthesis and generally distinguishes
A general protocol for staining cell for cytometry analysis
) 4.Cells stained with PI and SAv-FITC (no Annexin V-Biotin) Other Staining Controls: A cell line that can be easily induced to undergo apoptosis should be used to obtain positive control staining. It is important to note that the basal level
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