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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human PI4 kinase alpha. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX107441
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
0.64 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
PI4 kinase alpha
- 抗体英文名:
PI4 kinase alpha antibody [C1C2], Internal
- 抗体名:
PI4 kinase alpha 抗体 [C1C2], Internal
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
PI4 kinase alpha antibody [C1C2], Internal detects PI4 kinase alpha protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% PFA at RT for 15 min.
Green: PI4 kinase alpha protein stained by PI4 kinase alpha antibody [C1C2], Internal (GTX107441) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using PI4 kinase alpha(GTX107441) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI4 kinase alpha antibody [C1C2], Internal (GTX107441) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Gao Y et al., Cell Res 2022 (PMID:36280710)
Phosphatidylinositol 4-Kinase Assay in Ovarian Carcinoma Cells
). DAG stimulates protein kinase C and often promotes cell growth or differentiation. In case of PI, it is first phosphorylated by PI 3-kinase or PI 4-kinase. PI 3-kinase is often activated by phosphotyrosine of the activated growth factor receptors
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
-3-3 associates with several tyrosine-phosphorylated proteins and phosphatidylinositol 3-kinase (PI3-K) in T cells. We report here the identification of the 120-kDa 14-3-3tau-binding phosphoprotein present in activated T cell lysates as Cbl, a protooncogene
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