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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
Rat IgG2b, κ
- 形态:
液体
- 保存条件:
This product can be stored at 2-8°C for 12 months. Do not freeze.
- 克隆性:
Monoclonal
- 标记物:
None (Purified antibody-Azide Free/Low endotoxin)
- 适应物种:
Mouse
- 保质期:
12个月
- 目录编号:
E-AB-F12010
- 级别:
科研
- 库存:
999
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 宿主:
Rat
- 应用范围:
ICFCM;Neut;Stim
- 抗体英文名:
AF/LE Purified Anti-Mouse IL-2 Antibody[JES6-5H4]_货号:E-AB-F12010
- 抗体名:
AF/LE Purified Anti-Mouse IL-2 Antibody流式抗体[JES6-5H4]_货号:E-AB-F12010
- 规格:
500μg/50μg/1mg
| 规格: | 500μg | 产品价格: | ¥3160.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥760.0 |
| 规格: | 1mg | 产品价格: | ¥4760.0 |

AF/LE Purified Anti-Mouse IL-2 抗体流式抗体[JES6-5H4]产品名称:AF/LE Purified Anti-Mouse IL-2 Antibody[JES6-5H4], 货号:E-AB-F12010
| 产品描述: | IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes. |
| Alternate Names | Interleukin-2;IL-2;T-cell growth factor;TCGF;IL2; |
| Swissprot | P04351 |
| Gene ID | 16183 |
| Clone No | JES6-5H4 |
| Host | Rat |
| Reactivity | Mouse |
| Application | ICFCM;Neut;Stim |
| 同种型 | Rat IgG2b, κ |
| 同种型 Control | AF/LE Purified Rat IgG2b, κ 同种型 Control[LTF-2] |
| Conjugation | None (Purified antibody-Azide Free/Low endotoxin) |
| 储存方法 缓冲 | 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay. |
| 储存方法 | This product can be stored at 2-8°C for 12 months. Do not freeze. |
| Expiration date | 12个月 |
| Shipping | 冰袋 |

AF/LE Purified Anti-Mouse IL-2 抗体流式抗体[JES6-5H4]labscience®拥有先进的单抗,多抗和流式抗体开发技术平台,提供8500+高品质 抗体, 适用于胞内和胞外蛋白的鉴定、定量和定位研究。抗体已在多种应用中得到验证,包含 流式细胞术(Flow Cytometry)、 免疫印迹(WB)、 免疫组化(IHC/ICC)、 免疫(共)沉淀(IP/Co-IP)、 免疫荧光(IF) 等实验。抗体产品涉及多种生命科学研究领域,包括肿瘤、免疫、心血管、细胞生物学、神经科学、表观遗传学等。Elabscience®始终致力于提高抗体的质量和种类,以保障并加快您的研究进程
This product can be stored at 2-8°C for 12 months. Do not freeze.AF/LE Purified Anti-Mouse IL-2 抗体流式抗体[JES6-5H4]产品更多产品信息查看欢迎咨询我们和搜索武汉伊莱瑞特Elabscience官网查看。
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文献和实验Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
RPMI Medium 1640 with 2 mM L-Glutamin, 10% FCS/FBS and 100 U/ml penicillin/streptomycin can be used, or an equivalent culture medium. Heat inactivated Fetal Calf Serum (FCS). Recombinant mouse IL-2 (human IL-2 can also be used).
分别利用 CD3 与 CD28 的功能学抗体,以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤: 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体(克隆号:145-2C11)稀释至 5μg/ml,稀释后的抗体加入到 24 孔板中,每孔 400μl,4°C 包被过夜。(注:板子在加入抗体之前,先用无菌 PBS 润洗 2-3 遍,避免干孔); 1.2 小鼠脾脏淋巴细胞分离(次日) 1. 将 70μm
Intracellular Immunofluorescent Staining for Flow Cytometry
Brefeldin A JES6-5H4 IL-4 mouse spleen ConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d) 2d/3d anti-CD3 (10ug/ml immobilized) + anti-CD28 (2ug/ml soluble) 5hr
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